槟榔新拟盘多毛孢叶斑病菌的鉴定及生物学特性  

Identification and Biological Characteristics of the Pathogen Causing Neopestalotiopsis Leaf Spot Disease on Areca catechu

作　　者：李晶[1] 谢开泽 张超[1] 谢昌平[1] LI Jing;XIE Kaize;ZHANG Chao;XIE Changping(College of Plant Protection,Hainan University/Key Laboratory of Green Prevention and Control of Tropical Plant Diseases and Pests,Ministry of Education,Haikou,Hainan 570228,China)

机构地区：[1]海南大学植物保护学院/热带农林生物灾害绿色防控教育部重点实验室,海南海口570228

出　　处：《热带作物学报》2023年第8期1671-1678,共8页Chinese Journal of Tropical Crops

基　　金：海南省研究生创新科研课题(No.Qhys2021-57)。

摘　　要：为了确定引起槟榔叶斑病的病原菌及其生物学特性,从采集的病害标本上采用组织分离法对病原菌进行分离和纯化并对菌株的致病性进行测定,结合形态学特征和多基因(ITS-TUB-TEF)联合分析构建系统发育树对病原菌进行鉴定,并研究该病原菌在不同培养条件下的生物学特性。结果表明,引起槟榔叶斑病的病原菌为Neopestalotiopsis sonneratae。该病原菌菌丝在CA培养基上生长最快,在MEA培养基上气生菌丝最发达;菌丝生长和分生孢子萌发最适温度均为28℃;24 h光照条件最利于菌丝生长;菌丝生长最佳pH为5,分生孢子萌发最适pH为6。该研究结果可为槟榔叶斑病的防治提供理论依据。This study aimed to identify the pathogen which causes Areca catechu Linnaeus leaf spot and study its bio-logical characteristics.The pathogen was isolated and purified from disease samples by the tissue isolation method,and the pathogenicity of the strain was tested.Combined with morphological characteristics and multi gene(ITS-TUB-TEF)joint analysis,a phylogenetic tree was constructed to identify the pathogen,and to study its biological characteristics under different culture conditions.Leaf spot of A.catechu was caused by Neopestalotiopsis sonneratae.The hyphal growed the fastest on CA medium and the aerial hyphal was the most developed on MEA medium.The optimum tem-perature for both hyphal growth and spore germination was 28℃.24 h illumination was most favorable for the hyphal growth.Hyphal growed the best at pH 5,and the optimum pH for spore germination was 6.The study would provide a theoretical basis for the control of the leaf spot on A.catechu.

关 键 词：槟榔 叶斑病 新拟盘多毛孢 鉴定 生物学特性 

分 类 号：S435.671[农业科学—农业昆虫与害虫防治]