基于黄酮合成通路基因表达特性的木薯对二斑叶螨防御机理初探  

A Preliminary Study on the Defense Response of Cassava Cultivars to Tetranychus urticae Based on Gene Expression Pattern of Flavonoid Pathway

作　　者：乔阳 梁晓[2,3] 陈青 伍春玲[2,3] 刘迎 刘小强[2,3] 税军 姚晓文[1,2,3] QIAO Yang;LIANG Xiao;CHEN Qing;WU Chunling;LIU Ying;LIU Xiaoqiang;SHUI Jun;YAO Xiaowen(College of Plant Protection,Hainan University,Haikou,Hainan 570228,China;Environment and Plant Protection Institute,Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Pests Comprehensive Governance for Tropical Crops,Ministry of Ag-riculture and Rural Affairs/Hainan Engineering Research Center for Biological Control of Tropical Crops Diseases and Insect Pests,Haikou,Hainan 571101,China;Sanya Research Academy,Chinese Academy of Tropical Agricultural Sciences/Hainan Key Labo-ratory for Biosafety Monitoring and Molecular Breeding in Off-Season Reproduction Regions,Sanya,Hainan 572000,China)

机构地区：[1]海南大学植物保护学院,海南海口570228 [2]中国热带农业科学院环境与植物保护研究所/农业农村部热带作物有害生物综合治理重点实验室/海南省热带作物病虫害生物防治工程技术研究中心,海南海口571101 [3]中国热带农业科学院三亚研究院/海南省南繁生物安全与分子育种重点实验室,海南三亚572000

出　　处：《热带作物学报》2023年第8期1679-1688,共10页Chinese Journal of Tropical Crops

基　　金：国家木薯产业技术体系虫害防控岗位科学家专项(No.CARS-11-HNCQ);国家重点研发计划项目(No.2020YFD1000603);农业农村部农业资源调查与保护利用专项(No.NFZX-2021)。

摘　　要：木薯是全球重要的粮食作物和经济作物。二斑叶螨(Tetranychus urticae Koch)是重要的外来入侵生物和我国木薯主产区的四大有害生物之一。抗螨品种的选育与利用是实现害螨绿色综合防控的重要手段,作物中具有抗螨功能的次生代谢物质和其调控基因的挖掘与利用是培育抗性品种的有效途径。黄酮类次生代谢物质在作物抵御虫害中发挥重要作用,但其合成通路基因在木薯抗二斑叶螨中的功能尚不清楚。基于此,本研究分析抗、感螨木薯品种被二斑叶螨取食不同时间(1、4 d)后,黄酮合成通路关键基因表达量的变化情况。结果表明:与螨害前相比,感螨木薯品种面包、SC9和BAR900的CHS、PGT1、F3H、FLS、LAR、C3'H和CYP93B_16基因的相对表达量总体呈先降低(1 d)再升高(4 d)至螨害前(0 d)水平的趋势,而抗螨木薯品种C1115、SC9和缅甸中的上述基因相对表达量总体呈先显著升高(1d)再降低(4d)的趋势,但均显著高于螨害前(0d)的水平。进一步比较螨害前后抗、感螨木薯品种黄酮合成通路基因表达量的差异发现,二斑叶螨为害1 d后,抗螨木薯品种中CHS、PGT1、FLS和C3'H的表达量显著高于感螨木薯品种。相关性分析表明,这4个基因的表达量与木薯抗螨性呈显著正相关。螨害前(0d)和螨害4d后,抗螨木薯品种中上述基因的表达量总体也高于感螨木薯品种。该研究结果表明,抗螨木薯品种受螨害后,可能通过诱导黄酮合成基因的上调,从而增加具有抗虫功能的黄酮类代谢物含量以抵御二斑叶螨为害。该研究为深入阐明木薯抗螨分子机理、选育和创制抗螨木薯品种提供理论基础。Cassava is an important food crop and economic crop in the world.Tetranychus urticae is one of the important alien invasive pests and one of the four major cassava pests in China.Breeding and utilization of mite resistant varieties is an important means to realize green integrated pest control.The exploitation and utilization of secondary metabolites with mite resistance function and their regulation genes can effectively breed mite resistant varieties in crops.Flavonoid play an important role in plant defense against phytophagous pests,however,there is little knowledge about the function of flavonoid pathway genes in cassava resistance to mite.Based on this,this study used mite-resistant and mite-susceptible cassava cultivars were used to analyze the flavonoid pathway genes related to plant insect resistance and to analyze the difference of gene expression after mites fed for 1 d and 4 d,respectively.The results showed that,compared with before those mites fed,the relative expression of CHS,PGT1,F3H,FLS,LAR,C3'H and CYP93B_16 genes in the cassava cultivars of BREAD,SC9 and BAR900 showed a trend of first decreasing(1 d)and then increasing(4 d)to the levels before the mites feed(0 d),while the relative expression of the above genes in the mites-resistant cassava cultivars C1115,SC9 and Myanmar showed a trend of first significantly increasing(1 d)and then decreasing(4 d).However,they were all significantly higher than those of the levels before the mites feed(0 d).Further comparison of the differences in the expression of the flavonoids synthesis pathway gene in the mites-resistant and sensitive tapioca cultivars before and after mites was infested,it was found that after the pest of the two-spotted spider mites was harmed for 1 d,the expression of CHS,PGT1,FLS and C3'H in the mites-resistant cassava cultivars was significantly higher than that in the sensitive mites cassava varieties.Correlation analysis showed that the expression of the four genes was significantly and positively correlated with cassava mite resi

关 键 词：二斑叶螨 抗、感螨木薯品种 黄酮合成通路 基因表达 

分 类 号：S435.33[农业科学—农业昆虫与害虫防治]