基于微流动技术的病变红细胞流变特性的体外实验模拟  

In Vitro Experimental Simulation of Rheological Properties of Diseased Red Blood Cells Based on Microflow Technique

作　　者：陈凌峰 宋辉 李芬 CHEN Lingfeng;SONG Hui;LI Fen(College of Biomedical Engineering,Taiyuan University of Technology,Taiyuan 030024,China;College of Mechanical and Vehicle Engineering,Taiyuan University of Technology,Taiyuan 030024,China)

机构地区：[1]太原理工大学生物医学工程学院,太原030024 [2]太原理工大学机械与运载工程学院,太原030024

出　　处：《太原理工大学学报》2023年第5期898-904,共7页Journal of Taiyuan University of Technology

基　　金：国家自然科学基金资助项目(12002231,12172242)。

摘　　要：【目的】高血压、疟疾等疾病可引起血液中红细胞膜的刚性增加,其形态结构和流变特征也会发生改变,研究红细胞变形能力和其流动特征对这些疾病的临床诊断以及药物治疗等方面具有重要意义。【方法】采用一系列浓度的固定液处理红细胞以改变其变形能力获得病变红细胞流变特征,借助微流控芯片、注射泵、荧光倒置显微镜、高速摄像机等装置体外模拟病变红细胞在微血管中的流动特征,探索红细胞变形性能改变与无细胞层厚度、流动速度等参数之间的关系。【结果】经过固定液处理后的红细胞直径会增大;无细胞层厚度和流动速度会降低。这说明病变后的红细胞刚性增强,变形能力降低,其流动特征也会发生明显改变。【Purposes】Diseases such as hypertension and malaria infection often raise the rigidity of red blood cells(RBCs).Consequently,the morphology and rheological characteristics of RBCs change,which means understanding the deformability and flow characteristics of sick RBCs is of great significance for clinical diagnosis and drug treatment.【Methods】In this work,RBCs were treated with a series of fixatives to mimic sick RBCs.Devices such as microfluidic chip,injection pump,fluorescence inverted microscope,and high-speed camera were used to study the flow characteristics of treated RBCs flowing in microchannel in vitro.The relationships between the deformability of RBCs and Cell free Layer(CFL)thickness,flow velocity,and other parameters were explored.【Findings】The results show that,after treating with fixative solution,the diameter of RBCs increases,the width of CFL becomes thinner,and the velocity of RBCs becomes lower.After the disease,the rigidity of RBCs is enhanced,the deformability is reduced,and the flow characteristics of red blood cells are obviously changed.

关 键 词：红细胞 流动变形 微流动技术 无细胞层 速度分布 

分 类 号：R318.01[医药卫生—生物医学工程]