高温胁迫对丽蚜小蜂死亡率和保护酶系的影响  

Effect of high temperature stress on mortality and protective enzymes of Encarsia formosa

作　　者：龙佳芝 郭文秀 季敏 宋莹莹 李丽莉[2] 门兴元[2] 崔洪莹 于毅[2] 刘长仲 LONG Jia-zhi;GUO Wen-xiu;JI Min;SONG Ying-ying;LI Li-li;MEN Xing-yuan;CUI Hong-ying;YU Yi;LIU Chang-zhong(College of Plant Protection,Gansu Agricultural University,Lanzhou 730070,China;Institute of Plant Protec-tion,Shandong Academy of Agricultural Sciences,J inan 250100,China;Shandong Agriculture and Engineering University,Dezhou 253000,China)

机构地区：[1]甘肃农业大学植物保护学院,甘肃兰州7300701 [2]山东省农业科学院植物保护研究所,山东济南250100 [3]山东农业工程学院,山东德州253000

出　　处：《草原与草坪》2023年第4期130-136,共7页Grassland and Turf

基　　金：国家中药材产业技术体系建设专项基金项目(CARS-21);山东省农业科学院农业科技创新工程项目(CXGC2016B11)。

摘　　要：【目的】探究不同温度和时间处理对丽蚜小蜂(Encarsia formosa)的致死作用,明确丽蚜小蜂对温度胁迫的生理应答机制。【方法】测定不同温度(35、37和39℃)对丽蚜小蜂成虫24 h内存活率的影响和丽蚜小蜂成虫在35、37和39℃下胁迫4、8 h后体内的超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和过氧化物酶(POD)活性。【结果】丽蚜小蜂成虫的死亡率随着胁迫温度上升和时间的延长而加剧,在35℃以上1 h就出现死亡,与对照25℃存在显著差异。丽蚜小蜂成虫的SOD和POD活性随着温度的升高,呈现先升高后下降的趋势,在4 h时,SOD活性在37℃达到峰值,显著高于对照(25℃)的活性;POD活性在温度35℃、8 h时达到峰值且与其他温度和时间存在显著差异;CAT活性在37℃、4 h时达到最大值,并与其他温度和时间存在显著差异。SOD、POD和CAT 3种保护酶在35℃、8 h之内和37℃、4 h之内对丽蚜小蜂可起到保护的作用。【结论】温度高于35℃的环境对丽蚜小蜂的种群数量具有显著影响。【Objective】In order to explore the lethal effects of different temperatures and time duration on Encar⁃sia formosa,and to understand the physiological response mechanism of E.formosa to temperature stress.【Method】We recorded the survival rates of E.Formosa adults within 24 h at 35,37 and 39℃,as well as the changes of superox⁃ide dismutase(SOD),catalase(CAT)and peroxidase(POD)activities in vivo after 4h and 8h at 35,37 and 39℃.【Result】The mortality rate of the adults was aggravated with the increase of temperature and the prolongation of time.The death of E.Formosa adults was occurred in 1 hour above 35℃,which was significantly differed from that of the control at 25℃.With the increase of temperature,the SOD and POD activities in adults of E.formosa showed a trend of first increasing then decreasing.At 4 h,SOD activity peaked at 37℃,which was significantly higher than that of the control(25℃).POD activity peaked at 35℃at 8h and was significantly different from other treatments.CAT activity reached the maximum value at 37℃ at 4 h,and was significantly different from other treatments.SOD,POD and CAT can protect the E.formosa within 8h at 35℃ and 4 h at 37℃.【Conclusion】The temperature higher than 35℃ had a significant effect on the population of E.formosa.

关 键 词：丽蚜小蜂 高温胁迫 超氧化物歧化酶 过氧化物酶 过氧化氢酶 

分 类 号：S436.3[农业科学—农业昆虫与害虫防治]