基于戊二醛和非戊二醛处理体系制备的生物瓣膜材料的血液相容性评估  

Hemocompatibility of bioprosthetic heart valve materials respectively based on glutaraldehyde and non-glutaraldehyde treatment

作　　者：温晓晓 潘文志 张坤 潘孔荣 周达新 葛均波 WEN Xiaoxiao;PAN Wenzhi;ZHANG Kun;PAN Kongrong;ZHOU Daxin;GE Junbo(Peijia Medical Limited,Suzhou,215000,Jiangsu,P.R.China;Department of Cardiology,Zhongshan Hospital,Fudan University,Research Unit of Cardiovascular Techniques and Devices,Chinese Academy of Medical Sciences,Shanghai,200032,P.R.China)

机构地区：[1]沛嘉医疗科技(苏州)有限公司,江苏苏州215000 [2]复旦大学附属中山医院心内科中国医学科学院心血管技术与器械创新单元,上海200032

出　　处：《中国胸心血管外科临床杂志》2023年第9期1323-1328,共6页Chinese Journal of Clinical Thoracic and Cardiovascular Surgery

摘　　要：目的评估戊二醛处理体系与非戊二醛处理体系制备的牛心包生物瓣膜材料的血液相容性。方法新鲜牛心包剥离脂肪组织后采用戊二醛处理或非戊二醛处理。通过溶血试验、体外纤维蛋白原吸附、血小板黏附、凝血酶-抗凝血酶复合物(thrombin-antithrombin complex,TAT)检测、补体激活及半体内血液循环实验,评估两种生物瓣膜材料的血液相容性。结果溶血实验结果表明两种材料的溶血率均<5%。两种材料的TAT检测和补体激活实验结果与空白对照相比差异均无统计学意义。相比戊二醛处理,非戊二醛处理体系制备的牛心包生物瓣膜材料表现出明显降低的纤维蛋白原吸附、血小板黏附和血栓生成。结论相比戊二醛处理,非戊二醛处理体系制备的牛心包生物瓣膜材料具有较好的血液相容性。Objective To study the hemocompatibility of bioprosthetic heart valve materials respectively based on glutaraldehyde and non-glutaraldehyde treatment.Methods Fresh bovine pericardium was treated with glutaraldehyde or non-glutaraldehyde after adipose tissue was removed.To evaluate the hemocompatibility of the two bioprosthetic heart valve materials,hemolysis test,in vitro fibrinogen adsorption experiment,platelet adhesion experiment,thrombin-antithrombin complex(TAT)test,complement activation assay and ex vivo circulation experiment were performed.Results The hemolysis test results demonstrated that both of the materials showed hemolytic rates lower than 5%.The results of TAT test and complement activation assay showed no statistical differences among the two materials and the blank control group.Compared to the bioprosthetic heart valve materials with glutaraldehyde-based treatment,the materials with non-glutaraldehyde-based treatment showed significantly decreased fibrinogen adsorption,platelet adhesion and thrombosis.Conclusion Compared to the bioprosthetic heart valve materials with glutaraldehydebased treatment,the materials with non-glutaraldehyde-based treatment show better hemocompatibility.

关 键 词：生物瓣膜 牛心包 戊二醛 碳二亚胺 血液相容性 

分 类 号：R318.08[医药卫生—生物医学工程]