165份辣椒种质资源的PMMoV分子鉴定  

Molecular Identification of PMMoV in 165 Pepper Germplasm Resources

作　　者：陈建分 秦于玲[2] 刘子记[2] 王旭[1] 曹振木[2] 申龙斌[2] Chen Jianfen;Qin Yuling;Liu Ziji;Wang Xu;Cao Zhenmu;Shen Longbin(College of Horticulture,Hainan University,Haikou,570208;Tropical Crops Genetic Resources Institute,Chinese Academy of Tropical Agricultural Sciences,Haikou,571101)

机构地区：[1]海南大学园艺学院,海口570208 [2]中国热带农业科学院热带作物品种资源研究所,海口571101

出　　处：《分子植物育种》2023年第18期6042-6052,共11页Molecular Plant Breeding

基　　金：海南省基础与应用基础研究计划(自然科学领域)高层次人才项目(2019RC312)资助。

摘　　要：利用反转录聚合酶链式反应(RT-PCR)和致病型分析对165份辣椒种质资源进行分子鉴定。结果表明,在165份感病样品中均扩增得到PMMoV 220 bp的目的条带,阴性对照未扩增目的条带。测序结果表明,该序列为PMMo V的外壳蛋白(CP)基因,与已报道的各PMMoV分离物序列同源性达99.5%~100%。通过CP蛋白氨基酸序列分析及系统发育分析显示,该分离物与PMMoV P1,2致病型完全一致,确定辣椒园圃的PMMoV分离物为P1,2致病型。证实PMMoV已经在辣椒上发生危害,旨在为针对性地制定PMMoV的防控措施以及辣椒抗病毒品种的选育提供基础依据。Molecular identification of 165 pepper germplasm resources was performed using reverse transcrip-tion polymerase chain reaction(RT-PCR)and pathogenic type analysis.The results showed that the specific band of PMMoV 220 bp was amplified in all 165 susceptible samples,and the specific band was not amplified in the negative control.The sequencing results indicate that the sequence was the coat protein(CP)gene of PM-MoV with 99.5%~100%sequence homology with the reported PMMoV isolates.Furthermore,the CP protein amino acid sequence analysis as well as phylogenetic analysis confirmed that the isolate was completely consis-tent with the PMMoV P 1,2 pathogenic type,and the PMMoV isolate in the pepper gargen was determined as the P 1,2 pathogenic type.The damage of PMMoV to pepper was confirmed,aiming to provide the basis for the target-ed development of PMMoV prevention and control measures as well as the selection and breeding of virus-resis-tant varieties in pepper.

关 键 词：辣椒 辣椒轻斑驳病毒 RT-PCR检测 分子鉴定 

分 类 号：S641.3[农业科学—蔬菜学]