槟榔种苗、幼树AYLP及APV1的分子检测  

Molecular Detection of Arecanut Yellow Leaf Phytoplasam and Areca Palm Velarivirus 1 in Seedlings and Young Trees

作　　者：唐庆华[1] 林兆威 孟秀利 黄山春[1] 余凤玉[1] 宋薇薇[1] 覃伟权[1] Tang Qinghua;Lin Zhaowei;Meng Xiuli;Huang Shanchun;Yu Fengyu;Song Weiwei;Qin Weiquan(Hainan Engineering Research Center of Arecanut Industry,Coconut Research Institute of Chinese Academy of Tropical Agricultural Sciences,Wen-chang,571339)

机构地区：[1]中国热带农业科学院椰子研究所,海南省槟榔产业工程研究中心,文昌571339

出　　处：《分子植物育种》2023年第18期6065-6071,共7页Molecular Plant Breeding

基　　金：海南省重点研发项目(ZDYF2022XDNY208);海南省院士创新平台科研专项(YSPTZX202138);海南省院士创新平台资金(槟榔黄化病综合防控)项目共同资助。

摘　　要：由槟榔黄化植原体(areca palm yellow leaf phytoplasam, AYLP)引起的黄化病和槟榔隐症病毒1(areca palm velarivirus 1, APV1)引起的黄叶病毒病是海南槟榔生产中最重要的2种病害。为了明确2种病害的远距离传播途径,本研究采用Nested PCR和reverse transcription PCR (RT-PCR)方法对154份来源于万宁、琼海、屯昌和临高的槟榔种苗及幼树(2~4龄)样品进行了AYLP和APV1的分子检测。检测结果显示,部分种苗以及幼树叶片样品检测到AYLP (幼苗7份,幼树28份)或APV1 (幼苗15份,幼树48份),这表明这部分种苗和幼树已感染槟榔黄化病植原体或槟榔隐症病毒1。部分幼树样品(7份)同时检测到AYLP和APV1,表明该部分幼树被2种病原感染。本研究结果为槟榔黄化病和槟榔黄叶病毒病通过带毒种苗进行传播提供了实验证据。Yellow leaf disease(YLD)of arecanut and areca palm leaf yellowing virus disease(ALYVD)caused by arecanut yellow leaf phytoplasam(AYLP)and areca palm velarivirus 1(APV1)respectively are two of the most im-portant diseases in the industry in Hainan Province.In order to determine the long-distance transmission method,AYLP and APV1 were detected from 154 seedling and young trees(two to four years old)samples which collected from Wanning,Qionghai,Tunchang and Lingao by nested PCR and reverse transcription PCR(RT-PCR)respec-tively.The results showed that AYLP(7 seedlingsamples and 28 youngtree ones)or APV1(15 and 48 samples)was detected in the seedlings or young trees,which indicated that the seedlings and young trees from which the positive samples collected were infected by either AYLP or APV1.Both AYLP and APV1 were amplified in partial young tree samples(7 samples),which showed that these ones were infected by the two causal agents simultaneously.Our results gave a clue that YLD and ALYVD mayhave a remote transmission byareca palm seedlings.

关 键 词：槟榔黄化病 槟榔黄化植原体 槟榔黄叶病毒病 槟榔隐症病毒1 种苗检疫 

分 类 号：S763.7[农业科学—森林保护学]