烟草青枯病抗性的主基因+多基因混合遗传分析  

Genetic Analysis on Resistance to Bacterial Wilt of Tobacco by Mixture Model of Major Gene Plus Polygene

作　　者：方敦煌[1] 焦芳婵[1] 卢灿华 谢贺[1] 曾建敏[1] 陈学军[1] 童治军[1] Fang Dunhuang;Jiao Fangchan;Lu Canhua;Xie He;Zeng Jianmin;Chen Xuejun;Tong Zhijun(Key Laboratory of Tobacco Biotechnological Breeding,National Tobacco Genetic Engineering Research Center,Yunnan Academy of Tobacco Agri-cultural Sciences,Kunming,650021)

机构地区：[1]云南省烟草农业科学研究院,国家烟草基因工程研究中心,烟草行业烟草生物技术育种重点实验室,昆明650021

出　　处：《分子植物育种》2023年第18期6072-6079,共8页Molecular Plant Breeding

基　　金：中国烟草总公司项目[110202001024(JY-07)]和中国烟草总公司云南省公司项目(2020530000241008,20205-30000241009)共同资助。

摘　　要：烟草青枯病是一种破坏性极强的土传性细菌病害,可造成巨大的经济损失,并严重威胁烟草安全生产。为揭示烟草青枯病的抗性遗传规律,本研究以抗病品种‘岩烟97’、感病品种‘云烟87’为亲本配制杂交组合,连续2年采用主基因+多基因混合遗传模型方法对该组合P_(1)、P_(2)和F_(6)/F_(7)世代的青枯病抗性进行遗传分析。结果表明,‘岩烟97’的青枯病抗性表型值在烟草重组自交系群体(RILs,F6/F7)中均呈连续性正态分布,属于典型的数量性状遗传。‘岩烟97’青枯病抗性的最优遗传模型为4MG-AI,即受4对加性-上位性主基因模型控制,加性效应中以第1对主基因的加性效应值最大(14.751),上位性效应中以加性×加性上位性互作效应为主,主基因遗传效率为87.585%,表明‘岩烟97’的青枯病抗性遗传方式以主基因效应为主,环境效应影响较小。本研究结果为烟草抗青枯病主效基因的定位、克隆及紧密连锁标记的开发提供参考。Tobacco bacterial wilt is a highly destructive soil-borne bacterial disease of tobacco,which can cause huge economic losses and seriously threaten the safety of tobacco production.To explore the resistance inheritance of tobacco cultivar‘YY97'to bacterial wilt,a cross was made with susceptible/resistant tobaccos‘YY87'and‘YY97'as maternal and paternal parents,and applied the major gene plus polygene mixed genetic model method to analyze in the single generation(P_(1),P_(2) and F_(6)/F_(7)).The main research results showed that the phenotypic value of bacterial wilt resistance of'YY97 in RILs all showed unimodal normal distribution,which belonging to quantitative inheritance.The optimal resistance genetic model for‘Yanyan97'was 4MG-AI(i.e.,four addictive-epistatic major gene model).The addictive effect of the first pair major gene was largest(14.751),while additive×additive interaction effects were the dominant,and the genetic efficiency of the main genes was 87.585%.The results from two environments indicated that the inheritance of tobacco bacterial wilt resistance to‘YY97'was mainly controlled by major genes and less affected by the environment.The results of this study can provide reference for the mapping/cloning the major resistance genes and development of molecular markers related to disease resistance.

关 键 词：烟草 青枯病 主基因+多基因 遗传效应 

分 类 号：S435.72[农业科学—农业昆虫与害虫防治]