铁线莲花色相关次生代谢产物的测定及药理分析  

Determination and Pharmacological Analysis of the Color Related Secondary Metabolites in Clematis florida

作　　者：袁明霞[1] 宋微[1] 刘换换 马涛[1] 王皓洋 刘兰香 丁益 Yuan Mingxia;Song Wei;Liu Huanhuan;Ma Tao;Wang Haoyang;Liu Lanxiang;Ding Yi(Jiangsu Vocational College of Agriculture and Forestry,Jurong,212400;Jiangsu Jichuanglanhai Technology Co.,Ltd.,Suzhou,215000)

机构地区：[1]江苏农林职业技术学院,句容212400 [2]苏州极创澜海科技有限公司,苏州215000

出　　处：《分子植物育种》2023年第18期6134-6141,共8页Molecular Plant Breeding

基　　金：江苏省种业振兴揭榜挂帅项目(JBGS[2021]092);江苏省高校“青蓝工程”中青年学术带头人资助项目(苏教师函[2020]10号);江苏农林职业技术学院科技计划项目(2022kj36)共同资助。

摘　　要：为探明铁线莲花色相关次生代谢产物的含量及其药理,本研究应用Folin-酚比色法、香草醛-盐酸法、分光光度法等方法分别测定了7种铁线莲品种萼瓣中的总酚、单宁、原花青素、类黄酮和花色苷含量,且逐一对其药理进行了研究与分析。测定结果表明,7种铁线莲品种萼瓣中均积累了丰富的酚类物质和类黄酮物质,分别介于6.51~8.50 mg/g之间和1.15~3.04 mg/g之间,也均积累了一定量的单宁、原花青素和花色苷成分。药理分析表明,铁线莲花色相关次生代谢产物(总酚,单宁,原花青素,类黄酮和花色苷)均具有很好的抗氧化活性和清除自由基的能力,可以有效抗氧化、抗衰老、去除体内自由基、保护心脑血管、缓解炎症、抑制癌症等。本研究的实施为探明铁线莲花色相关次生代谢产物的药用价值奠定了基础,亦为未来的药用开发提供了一定的理论依据。In order to find out the content and pharmacological effects of the color related secondary metabolites in Clematis florida,methods such as Folin-phenol method,vanillin-hydrochloric acid method,spectrophotometry were used to determine the content of total phenols,tannins,proanthocyanidins,flavonoids and anthocyanins in the calyx petals of 7 Clematis florida varieties,and their pharmacological effects were studied and analyzed one by one.The results showed that 7 Clematis florida varieties all accumulated abundant phenols and flavonoids in their calyx petals,with 6.51~8.50 mg/g and 1.15~3.04 mg/g,respectively,and also all accumulated a certain amount of tannins,proanthocyanidins and anthocyanins.The pharmacological analysis showed that the color related secondary metabolites(total phenols,tannins,proanthocyanidins,flavonoids and anthocyanins)in Clematis florida all have good antioxidant activity and the ability to remove free radicals,which can effectively resist oxidation,anti-aging,remove free radicals in the body,protect cardiovascular and cerebrovascular systems,alleviate inflammation,in-hibit cancer,etc.The implementation of this study has laid a foundation for ascertaining the medicinal value of the color related secondary metabolites in Clematis florida,and also provided a theoretical basis for pharmaceutical de-velopment of secondary metabolites in the future.

关 键 词：铁线莲属 次生代谢产物 药理作用 

分 类 号：S567.239[农业科学—中草药栽培]