A controllable self-localized imaging strategy capable of synchronous in situ tracking of local changes in intracellular bioactive small-molecules  

作　　者：Ke Li Shuai Xu Benhua Wang Shuangyan Huan Xiangzhi Song Lin Yuan Xiao-Bing Zhang 

机构地区：[1]State Key Laboratory of Chemo/Biosensing and Chemometrics,College of Chemistry and Chemical Engineering,Hunan University,Changsha,410082,China [2]College of Chemistry&Chemical Engineering,Central South University,Changsha,410083,China [3]School of Chemistry and Chemical Engineering,University of Jinan,Jinan,250022,China

出　　处：《Science China Chemistry》2023年第8期2425-2434,共10页中国科学（化学英文版）

基　　金：supported by the National Natural Science Foundation of China(22234003,22074036,22204177);the Special Funds for the Construction of Innovative Provinces in Hunan Province(2021RC4021);the Fundamental Research Funds for the Central Universities。

摘　　要：In situ tracking and localization of ubiquitous bioactive small molecules(BSMs)within their native habitats is particularly challenging because of their low-molecular weight and widespread distribution properties.We report the proof of concept of a synchronous in situ imaging strategy,whereby the representative BSM amino-biothiols(ABs)mediate activation of the selflocalizable probe HYPQS,thereby releasing insoluble emissive precipitates to afford holistic distribution information of ABs.Notably,three organelle-targetable ABs inhibitors were innovatively fabricated for directed clearance of ABs in particular organelles,providing a powerful aid for HYPQS to achieve programmed in situ tracking of ABs in different organelles“on demand”.Biological transmission electron microscopy images confirmed that this probe released insoluble emissive precipitates at the reaction sites,which is of primary importance for achieving synchronous in situ tracking of BSMs.Furthermore,the probe HYPQS was successfully applied to monitor the dynamic changes in the endogenous ABs pool during diverse cell events.This strategy opens a promising avenue for investigating the undiscovered functional mechanism of local BSMs in relevant biological processes.

关 键 词：bioactive small-molecules in situ imaging fluorescent probes precipitated fluorophore imaging reagents 

分 类 号：Q2-33[生物学—细胞生物学]