基于UPLC-Q-TOF/MS技术的大建中汤血清药物化学研究  

Serum pharmacochemistry study of Dajianzhong Decoction based on UPLC-Q-TOF/MS

作　　者：蒋淼[1] 彭伟[1] 吴纯洁[1] 王家葵[1] JIANG Miao;PENG Wei;WU Chun-jie;WANG Jia-kui(Key Laboratory of Standardization of Chinese Herbal Medicine,Ministry of Education,State Key Laboratory of Southwestern Characteristic Chinese Medicine Resources,School of Pharmacy,Chengdu University of Traditional Chinese Medicine,Chengdu 611137,China)

机构地区：[1]成都中医药大学药学院,中药标准化教育部重点实验室,西南特色中药资源国家重点实验室,四川成都611137

出　　处：《中草药》2023年第16期5154-5164,共11页Chinese Traditional and Herbal Drugs

基　　金：四川省中医药管理局2020年度花椒药用价值研发专项(2020HJZX001);成都市知识产权局专项(202000092)。

摘　　要：目的应用血清药物化学方法分析大建中汤入血成分及体外化学成分。方法采用UPLC-Q-TOF/MS技术快速鉴定大建中汤经大鼠ig给药后吸收入血的原型成分、代谢成分以及体外化学成分,结合UNIFI软件系统分析,根据质谱相对分子质量、裂解规律、文献报道等方法对质谱碎片信息进行分析,表征大建中汤入血成分及体外化学成分。结果在给药血清中检测出22个入血成分,包括5个原型成分和17个代谢产物,体外分析鉴定得到41个化学成分。结论UPLC-Q-TOF/MS技术可实现大建中汤体内外化学成分的快速鉴定,且血清中鉴定出的原型成分和代谢产物可能是大建中汤潜在的活性成分,可为其质量控制研究提供依据。Objective To analyze the blood components and in vitro chemical components of Dajianzhong Decoction(大建中汤)by serum pharmacochemistry method.Methods UPLC-Q-TOF/MS technology was used to rapidly identify the prototype components,metabolic components and in vitro chemical components absorbed into the blood of Dajianzhong Decoction after oral administration to rats.Combined with UNIFI software,the molecular weight of mass spectrometry,mass spectrometry fragmentation rules,literature reports and other methods were used to analyze the mass spectrometry fragmentation information to characterize the blood components and in vitro chemical components of Dajianzhong Decoction.Results A total of 22 blood components were identified in the administered plasma,including five prototype components and 17 metabolites,and 41 chemical components were identified by in vitro analysis.Conclusion UPLC-Q-TOF/MS technology can realize the rapid identification of chemical components in Dajianzhong Decoction in vitro and in vivo,and the prototype components and metabolites identified in serums may be the potential active components of Dajianzhong Decoction,which can be used for quality control studies.

关 键 词：大建中汤 血清药物化学 UPLC-Q-TOF/MS 原型成分 代谢产物 人参皂苷RG1 羟基-α-山椒素 

分 类 号：R284.1[医药卫生—中药学]