基于活性成分比例一致性的白芷药材质量评价数据挖掘  

Data mining for quality evaluation of Angelica dahuricaherbs based on consistency of active ingredient proportions

作　　者：孙国志 段宝忠 黄文昊 张晓丹[1] 杨宗岐[1] 梁宗锁[1,3] 杨东风 SUN Guo-zhi;DUAN Bao-zhong;HUANG Wen-hao;ZHANG Xiao-dan;YANG Zong-qi;LIANG Zong-suo;YANG Dong-feng(Key Laboratory of Plant Secondary Metabolism and Regulation in Zhejiang Province,College of Life Sciences and Medicine,Zhejiang Sci-Tech University,Hangzhou 310018,China;College of Pharmaceutical Science,Dali University,Dali 671003,China;Shaoxing Biomedical Research Instituteof Zhejiang Sci-Tech University Co.,Ltd,Zhejiang Engineering Research Center for the Development Technology of Medicinal and Edible Homologous Health Food,Shaoxing 312075,China)

机构地区：[1]浙江理工大学生命科学与医药学院,浙江省植物次生代谢调控重点实验室,浙江杭州310018 [2]大理大学药学院,云南大理671003 [3]浙江理工大学绍兴生物医药研究院有限公司,药食同源保健食品开发技术浙江省工程研究中心,浙江绍兴312075

出　　处：《中草药》2023年第16期5329-5338,共10页Chinese Traditional and Herbal Drugs

基　　金：浙江省自然科学基金杰出青年基金项目(LR21H280002);浙江省“十四五”育种专项中药材协作组项目(2021C02074);中央本级重大增减支项目子课题(2060302);延安大学博士启动项目(YDBK2019-64)。

摘　　要：目的白芷药材有效成分多且复杂,单一质量标准或者单一成分控制难以有效地衡量白芷药材的质量。通过探究白芷药材有效成分比例一致性,对白芷药材的质量进行评价和控制。方法提取78篇文献中的1297个白芷药材样本中的有效成分:欧前胡素、异欧前胡素、氧化前胡素、佛手柑内酯、水合氧化前胡素、白当归素、花椒毒酚的含量数据。对符合《中国药典》质量标准的1118个样本进行各有效成分含量比例一致性分析。结果发现各个香豆素类有效成分与标志性成分欧前胡素含量比值在一定范围内波动,例如异欧前胡素和欧前胡素含量比值在0.08~1.08,氧化前胡素和欧前胡素含量比值在0.02~2.00,佛手柑内酯和欧前胡素含量比值0.02~0.29,含量比值在范围之外的白芷药材一般为不合格药材。然后将各个香豆素类有效成分与标志性成分欧前胡素含量比值作正态分布曲线,发现各含量比值以均数为中心呈正态分布,例如异欧前胡素和欧前胡素含量比值以0.451为中心,氧化前胡素和欧前胡素含量比值以0.719为中心,佛手柑内酯和欧前胡素含量比值以0.0903为中心,越靠近中心则含量比值越稳定,药材质量越好。结论单一的外观性状鉴别并不能全面反映出白芷药材质量,甚至白芷药材中的有效成分含量与按外观划分的等级相反,因此可以从有效成分的种类、组分比例和含量3个角度准确评价白芷药材质量,通过兼顾外观性状指标和化学质量指标,综合评价白芷药材的规格等级。ObjectiveThe active ingredients of Angelica dahuricaherbs are many and complex,and it is difficult to measure the quality of A.dahuricaherbs effectively by a single quality standard or a single component control.This paper evaluates and controls the quality of A.dahuricaherbs by exploring the consistency of the proportions of the active ingredients of A.dahuricaherbs.Methods Mining the content data of 1297 A.dahuricaherb samples from 78 literatures for the active ingredients:imperatorin,isoimperatorin,oxypeucedanin,bergapten,oxypeucedanin hydrate,byakangelicin,xanthotoxol.The 1118 samples that met the quality standards of the Chinese Pharmacopoeiawere analyzed for the consistency of the proportion of the content of each active ingredient.ResultsIt was found that the content ratio of each coumarin active ingredient and the iconic ingredient imperatorin fluctuated within a certain range,for example,the content ratio of isoimperatorin and imperatorin was between 0.08 and 1.08,the content ratio of oxypeucedanin to imperatorin was between 0.02 and 2.0,and the content ratio of bergapten toimperatorin was between 0.02 and 0.29.A.dahuricaherbs whose content ratio was outside the range were generally unqualified herbs.Then,the ratio of the content of each coumarin active ingredient to the iconic ingredient imperatorin was drawn as a normal distribution curve.It was found that the content ratios were normally distributed with the mean as the center,for example,the content ratio of isoimperatorin and imperatorin was centered at 0.451,the content ratio of oxypeucedanin and imperatorin was centered at 0.719,and the content ratio of bergapten and imperatorin was centered at 0.0903.The closer to the center,the more stable the content ratio,and the better the quality of the herbs.ConclusionSingle appearance traits identification does not fully reflect the quality of A.dahuricaherbs,and even the content of active ingredients in A.dahuricaherbs is opposite to the grades according to the appearance,so the quality of A.dahuricaherbs

关 键 词：白芷 质量评价 含量比例 欧前胡素 异欧前胡素 氧化前胡素 佛手柑内酯 水合氧化前胡素 白当归素 花椒毒酚 

分 类 号：R286[医药卫生—中药学]