金属有机框架与大肠杆菌杂合体光驱产氢研究  

Light-driven hydrogen production study of a Metal-Organic Framework-Escherichia coli biohybrid system

作　　者：李佳璐 沈俊峰 曾翠平 侯燕萍 王博 LI Jia-lu;SHEN Jun-feng;ZENG Cui-ping;HOU Yan-ping;WANG Bo(School of Resources,Environment and Materials,Guangxi University,Nanning 530004,China;Shenzhen Institute of Advanced Technology,Chinese Academy of Sciences,Shenzhen 518005,China)

机构地区：[1]广西大学资源环境与材料学院,广西南宁530004 [2]中国科学院深圳先进技术研究院,广东深圳518005

出　　处：《湖北师范大学学报（自然科学版）》2023年第3期82-89,共8页Journal of Hubei Normal University：Natural Science

基　　金：国家自然科学基金青年科学基金项目(22008252)。

摘　　要：基于内消旋-(4-羧基苯基)卟吩及氯化锆合成了金属有机框架PCN-222,并对其进行了系统的形貌与物性表征。通过正负电荷相互吸引使大肠杆菌聚集于PCN-222表面,构建了杂合体系。紫外可见漫反射结果证实PCN-222具有较强的可见光吸收性能,光电流测试与电化学阻抗测试均证实PCN-222具有优异的光电转化活性。光致发光光谱测试证明大肠杆菌可接收PCN-222表面产生的光电子。在光照下结合有PCN-222的大肠杆菌产氢性能显著提升。胞内NADH测试结果进一步证实大肠杆菌接收的光电子转化为过剩的还原力,进而以氢气的形式释放,揭示了光驱产氢机理。This paper synthesized a PCN-222 Metal-Organic Framework based on tetrakis(4-carboxyphenyl)porphyrinand ZrCl 4,which has systematical morphology and physical properties.In addition it constructed the MOF-Escherichia.coli biohybrid system through electrostatic adsorption of the bacteria on the surface of PCN-222.UV-VIS diffuse reflection proved that PCN-222 has strong visible-light absorption performance,and the photocurrent and electrochemical impedance tests both confirmed that PCN-222 has excellent photoelectric conversion activity.Photoluminescence spectroscopy tests indicated that E.coli can receive photoelectrons produced by PCN-222 and the hydrogen production performance of E.coli combined with PCN-222 was significantly improvedunder light.The elevated intracellular NADH further proved that the photoelectrons received by E.coli could be converted into excess reducing power,which was released in the form of hydrogen,revealing the mechanism of light-driven hydrogen production.

关 键 词：金属有机框架 大肠杆菌 半人工光合作用 氢气 

分 类 号：O631[理学—高分子化学]