应用SSR分子标记鉴定非洲菊F1代杂种的真实性  

Authentication of F1 Gerbera jamesonii Hybrids Using SSR Molecular Markers

作　　者：曹奕鸯 夏朝水 陈玮婷 甘玮欣 林辉锋 林发壮 周辉明 CAO Yiyang;XIA Chaoshui;CHEN Weiting;GAN Weixin;LIN Huifeng;LIN Fazhuang;ZHOU Huiming(Flower Research Institute,Institute of Sanming Agricultural Sciences,Sanming,Fujian 365051,China;Fujian Key Laboratory of Crop Genetic Improvement and Innovative Utilization for Mountain Area,Sanming Academy of Agricultural Sciences,Sanming,Fujian 365051,China)

机构地区：[1]三明市农业科学研究院花卉研究所,福建三明365051 [2]福建省(山区)作物遗传改良与创新利用重点实验室,福建三明365051

出　　处：《福建农业学报》2023年第7期842-850,共9页Fujian Journal of Agricultural Sciences

基　　金：福建省科技计划农业引导性项目(2021N0042);福建省科技计划星火项目(2022S2023)。

摘　　要：【目的】应用SSR分子标记技术鉴定非洲菊(Gerbera jamesonii Bolus)F1代杂种的真实性,以期为非洲菊新品种(系)杂种的真实性鉴定提供方法。【方法】选用非洲菊罗德里、热带草原及其65个正反交F1代杂种,部分自主选育非洲菊新品种(系)及其亲本为材料,从非洲菊转录组中发掘的65对SSR引物中筛选条带清晰、重复性好、亲本间无相同等位基因位点的引物,运用于非洲菊F1代杂种的真实性鉴定。【结果】65对SSR引物中,有26对引物在非洲菊亲本罗德里、热带草原间具有多态性,多态性比例达40%,其中,g24、g32、g38、g64这4对引物扩增非洲菊亲本间无相同等位基因位点,条带清晰可辨,扩增正反交后代都包含双亲的各一条带,都能将65个正反交后代鉴定为真杂种。引物g24、g04、g44、g39扩增非洲菊新品种(系)均包含双亲的各一条带,可分别将非洲菊新品种(系)明卉粉黛、明卉红颜、魅粉、幻彩鉴定为真杂种。【结论】SSR分子标记在非洲菊亲本中多态性丰富,可应用于非洲菊杂交后代真实性鉴定,能够有效辅助非洲菊新品种鉴定和选育。【Objective】SSR molecular markers were used to verify the authenticity of F1 hybrids of Gerbera jamesonii Bolus.[Methods]From Rodrigo,Savannah,and their progenies,65 reciprocal F1 hybrids of gerbera,primers with clear bands,high repeatability,and free of identical allele between parents were selected.Based on the transcriptomes,applicable means of species authentication were investigated.【Results】Of the 65 selected pairs of SSR primers,26 were polymorphic at a rate of 40%.No identical alleles between the parents were found on the g24,g32,g38,and g64 primers with clear and distinct bands.They all contained a band of each parent in their amplified positive and negative F1 progenies and could correctly distinguish true from false hybrids.Meanwhile,the primer g24,g04,g44,and g39 could amplify new gerbera varieties/strains and also contained a band of each parent.However,they were limited to positively identifying genuine hybrids of Minghuifendai,Minghuihongyan,Meifen,and Huancai.【Conclusion】The SSR molecular markers selected could satisfactorily be used to authenticate the F1 hybrids of G.jamesonii Bolus for plant selection and commercial identification.

关 键 词：非洲菊 SSR分子标记 杂种真实性鉴定 

分 类 号：S682.1[农业科学—观赏园艺]