鹤顶兰种子的真菌共生萌发研究  

Study on Fungi Symbiotic Germination of Phaius tancarvilleae Seeds

作　　者：汪雨 高鑫祯 邵士成[2] 王晓静[2] 罗艳[2] 马长乐[1,3] WANG Yu;GAO Xinzhen;SHAO Shicheng;WANG Xiaojing;LUO Yan;MA Changle(School of Landscape Architecture,Southwest Forestry University,Kunming 650224,China;Xishuangbanna Tropical Botanical Garden,Chinese Academy of Sciences,Mengla Yunnan 666303,China;Southwest Landscape Architecture Engineering Technology Research Center,State Forestry and Grassland Administration,Kunming 650224,China;University of Chinese Academy of Sciences,Beijing 100049,China)

机构地区：[1]西南林业大学园林园艺学院,昆明650224 [2]中国科学院西双版纳热带植物园,云南勐腊666303 [3]国家林业和草原局西南风景园林工程技术研究中心,昆明650224 [4]中国科学院大学,北京100049

出　　处：《种子》2023年第7期1-9,56,共10页Seed

基　　金：国家自然科学基金(32270225);中国科学院西双版纳热带植物园园林园艺中心研究基金(E2ZK291B09)。

摘　　要：采用原地共生萌发技术获得了鹤顶兰的原球茎,从中分离和筛选出了一株适合的共生菌——胶膜菌(HDL-2)。为验证分离菌株对鹤顶兰种子萌发的有效性,将HDL-2菌株与鹤顶兰种子在燕麦培养基上进行共生萌发试验。经过大约110 d的培养,HDL-2菌株可明显促进鹤顶兰种子萌发、形成原球茎并最终分化成幼苗,平均萌发率为(86.69±3.19)%,与无菌萌发相比有较高的萌发效率。表明分离得到的胶膜菌能促进鹤顶兰的种子萌发和原球茎发育。鹤顶兰种子实现共生萌发对于鹤顶兰资源的种质保育、人工栽培和可持续利用均具有重要意义。Using in situ symbiotic germination technology to obtain the protocorm of P.tancarvilleae,from which one highly compatible fungus(HDL-2)strain,Tulasnella sp.was isolated and screened out.In order to verify the effect of the isolated fungal strains on seed germination,seeds of P.tancarvilleae were co-cultured with the HDL-2 strain in an oatmeal agar medium.The HDL-2 enhanced seed germination and promoted protocorm growth and finally differentiated into seedlings after 110 days,and the average germination rate was(86.69±3.19)%.Compared with asymbiotic seed germination,seed germination promoted by HDL-2 had higher germination rate.Results showed that the isolated fungal strain HDL-2 could promote seed germination and protocorm growth of P.tancarvilleae.The success of symbiotic germination of P.tancarvilleae was of great importance for its germplasm conservation,artificial production and sustainable utilization.

关 键 词：鹤顶兰 种子 萌发真菌 共生萌发 

分 类 号：S682.31[农业科学—观赏园艺]