玉米穗部性状QTL定位与候选基因分析  

QTL Mapping and Candidate Gene Analysis of Ear Traits in Maize

作　　者：聂蕾 何玥 郭爽 王栋 涂亮 刘鹏飞 王安贵[2] 祝云芳[2] 吴迅 陈泽辉[2] 郭向阳[2] NIE Lei;HE Yue;GUO Shuang;WANG Dong;TU Liang;LIU Pengfei;WANG Angui;ZHU Yunfang;WU Xun;CHEN Zehui;GUO Xiangyang(Agricultural College of Guizhou University,Guiyang 550025,China;Institute of Upland Food Crops,Guizhou Academy of Agricultural Sciences,Guiyang 550006,China;Ministry of Agriculture and Rural Affairs Key Laboratory of Crop Genetic Resources and Germplasm Innovation in Karst Region,Guiyang 550006,China)

机构地区：[1]贵州大学农学院,贵阳550025 [2]贵州省农业科学院旱粮研究所,贵阳550006 [3]农业农村部喀斯特山区作物基因资源与种质创新重点实验室,贵阳550006

出　　处：《种子》2023年第7期24-30,共7页Seed

基　　金：贵州省科技支撑计划项目(黔科合支撑[2022]重点025);贵州省科技支撑计划(黔科合支撑[2022]重点026);贵州省科技支撑计划(黔科合支撑[2022]重点029);国家自然科学基金项目(32171981、32060460、32160451);贵州省科技基金(黔科合基础[2020]1Z019);贵州省农业科学院科技创新项目([2022]03号);贵州省育种科研基础平台创新能力建设(黔科合服企[2022]014);贵州省自然科学基金项目(黔科合基础[2022]一般236);贵州科技计划项目(黔科合基础-ZK[2023]一般173);黔农科院国基后补助[2021]55号;黔农科院国基后补助[2022]02号。

摘　　要：本研究以热带玉米骨干自交系T32和QR273为亲本构建的150份F_(2)、F_(2∶3)分离家系为材料,利用简化基因组测序技术(GBS)对F_(2)单株的基因型鉴定,同时分别在甘肃张掖和贵州贵阳两环境条件下对F_(2∶3)家系的穗长、穗粗、穗行数、行粒数和秃尖长等5个穗部相关性状进行表型评价,采用完备区间作图法进行QTL定位。结果表明,两个环境下共检测到46个穗部相关性状QTL,分布于10条染色体上,可分别解释表型变异范围为1.89%~17.18%,可解释穗部性状表型变异大于10%的QTL有6个。结合公共数据库,利用生物信息学分析策略,预测出6个控制穗部性状变异的候选基因(Zm00001d041072,Zm00001d053048,Zm00001d011355,Zm00001d041073,Zm00001d030086,Zm00001d030088),这些基因所编码的蛋白具有植物生长发育、激素合成、营养成分转运和其他许多生物学功能,可作为后续基因图位克隆和分子辅助育种的候选靶标。In this study,150 F_(2),F_(2∶3) offsprings crossed by using tropical maize inbred lines of T32 and QR273 as parents,were selected as materials,and genotypes of F_(2) individuals were done by using simplified genome sequencing(GBS)method.While five ear related traits of the ear length(cm),ear diameter(cm),ear row number(row),grain number per row(grain)and ear barren tip(cm)of F_(2∶3) families were evaluated under two environments of Zhangye,Gansu and Guiyang,Guizhou.QTL mapping was performed by using the complete interval mapping method.Results showed that,a total of 46 QTL associated with ear-related traits were identified under two environments,distributed on 10 Chl omosomes and explained 1.89%-17.18%of the phenotypic variation,respectively.Six QTL explaining more than 10% of the phenotypic variation of ear traits were identified in this paper.And six candidate genes controlling ear related traits were identified including Zm00001d041072,Zm00001d053048,Zm00001d011355,Zm00001d041073,Zm00001d030086 and Zm00001d030088,which encoded proteins involved in plant growth and development,hormone synthesis,nutrient transport and other biological functions,of which these candidate genes could be used as candidate targets for gene cloning and molecular assisted breeding in the future.

关 键 词：玉米 穗部性状 QTL定位 候选基因 

分 类 号：S513[农业科学—作物学]