金毛狗脊原叶体增殖和孢子体转化条件的优化  

Condition Optimization for Prothallus Proliferation and Sporophyte Transformation in Cibotium barometz

作　　者：黄嘉凤 但俊 黄满霖 陈选鹏 黄海波[1] HUANG Jiafeng;DAN Jun;HUANG Manlin;CHEN Xuanpeng;HUANG Haibo(College of Traditional Chinese Medicine,Guangzhou University of Chinese Medicine,Guangzhou 510006,China)

机构地区：[1]广州中医药大学中药学院,广州510006

出　　处：《种子》2023年第7期84-90,共7页Seed

基　　金：广东省省级乡村振兴战略(农业科技创新及推广体系建设)专项——广东省现代南药产业技术体系创新团队(2022KJ148);广东省普通高校“服务乡村振兴计划”重点领域专项——基于广东乡村振兴的南药产业科技服务体系构建(2019KZDZX2017);广东省中药材规范化生产技术——人才培养专项(E1-6205-17-112-004)。

摘　　要：为探究不同浓度的无机盐、蔗糖、KT、NAA和6-BA等对金毛狗脊原叶体增殖和孢子体转化的影响,采用正交设计实验方法,设置5因素4水平正交试验,筛选金毛狗脊原叶体增殖和孢子体转化的最佳培养基。结果表明,原叶体增殖最佳培养基为1/2MS+2%蔗糖+0.1 mg/L NAA+0.3 mg/L 6-BA+0.1 mg/L KT。孢子体转化最佳培养基为1/4MS+1%蔗糖+0.5 mg/L KT+0.1 mg/L NAA。The effects of different concentrations of inorganic salts,sucrose,KT,NAA and 6-BA on the prothallus proliferation and sporophyte transformation in Cibotium barometz were investigated.The orthogonal design experimental method was used to set four levels of the above five factors to screen the best medium for the prothallus proliferation and sporophytes transformation in Cibotium barometz.Results showed that the optimal medium for prothallus proliferation was 1/2MS+2% sucrose+0.1 mg/L NAA+0.3 mg/L 6-BA+0.1 mg/L KT and the optimal medium for sporophytes transformation was 1/4MS+1%sucrose+0.5 mg/L KT+0.1 mg/L NAA.

关 键 词：金毛狗脊 原叶体增殖 孢子体转化 

分 类 号：S567.239[农业科学—中草药栽培]