ZmMS1/ZmLBD30-orchestrated transcriptional regulatory networks precisely control pollen exine development  

作　　者：Quancan Hou Xueli An Biao Ma Suowei Wu Xun Wei Tingwei Yan Yan Zhou Taotao Zhu Ke Xie Danfeng Zhang Ziwen Li Lina Zhao Canfang Niu Yan Long Chang Liu WeiZhao FeiNi Jinping Li Daolin Fu Zhong-NanYang Xiangyuan Wan 

机构地区：[1]Research Institute of Biology and Agriculture,University of Science and Technology Beijing,Beijing 100083,China [2]Beijing Engineering Laboratory of Main Crop Bio-Tech Breeding,Zhongzhi International Institute of Agricultural Biosciences,Beijing 100192,China [3]State Key Laboratory of Crop Biology,College of Agronomy,Shandong Agricultural University,Taian,Shandong 271018,China [4]Shanghai Key Laboratory of Plant Molecular Sciences,College of Life Sciences,Shanghai Normal University,Shanghai 200234,China

出　　处：《Molecular Plant》2023年第8期1321-1338,共18页分子植物（英文版）

基　　金：supported by the National Key Research and Development Program of China(2022YFF1003500 to X.Wan,2022YFF1002400 and 2021YFF1000302 to S.W.,and 2022YFF1100501 to X.Wei);the National Natural Science Foundation of China(31771875 to X.Wan,31900610 to Q.H.31971958 to X.A.,and 31871702 to S.W.);the Fundamental Research Funds for the Central Universities of China(06500136 to X.Wan);the Beijing Nova Program(Z201100006820114 to Q.H.).

摘　　要：Because of its significance for plant male fertility and,hence,direct impact on crop yield,pollen exine development has inspired decades of scientific inquiry.However,the molecularmechanismunderlying exine formation and thickness remains elusive.In this study,we identified that a previously unrecognized repressor,ZmMS1/ZmLBD30,controls proper pollen exine development in maize.Using an ms1 mutant with aberrantly thickened exine,we cloned a male-sterility gene,ZmMs1,which encodes a tapetum-specific lateral organ boundary domain transcription factor,ZmLBD30.Weshowed thatZmMs1/ZmLBD30 is initially turned on by a transcriptional activation cascade of ZmbHLH51-ZmMYB84-ZmMS7,and then it serves as a repressor to shut down this cascade via feedback repression to ensure timely tapetal degeneration and proper level of exine.This activation-feedback repression loop regulating male fertility is conserved in maize and sorghum,and similar regulatory mechanism may also exist in other flowering plants such as rice and Arabidopsis.Collectively,these findings reveal a novel regulatory mechanism of pollen exine development by which a long-sought master repressor of upstream activators prevents excessive exine formation.

关 键 词：ZmMs1/ZmLBD30 LBD transcription factor feedback repression EXINE male sterility MAIZE 

分 类 号：S513[农业科学—作物学]