Phytochemical Estimation and Therapeutic Amelioration of Aesculus hippocastanum L. Seeds Ethanolic Extract in Gastric Ulcer in Rats Possibly by Inhibiting Prostaglandin Synthesis  

作　　者：Sahar Idris Anuradha Mishra Mohammad Khushtar 

机构地区：[1]Faculty of Pharmacy,Integral University,Dasauli,Lucknow,226026,Uttar Pradesh,India [2]Amity Institute of Pharmacy,Lucknow,Amity University Uttar Pradesh,Noida,201313,India

出　　处：《Chinese Journal of Integrative Medicine》2023年第9期818-824,共7页中国结合医学杂志（英文版）

摘　　要：Objective To quantify phytochemicals using liquid chromatography and mass spectroscopy(LCMS)analysis and explore the therapeutic effect of Aesculus hippocastanum L.(AH)seeds ethanolic extract against gastric ulcers in rats.Methods Preliminary phytochemical testing and LCMS analysis were performed according to standard methods.For treatment,the animals were divided into 7 groups including normal control,ulcer control,self-healing,AH seeds low and high doses,ranitidine and per se groups.Rats were orally administered with 10 mg/kg of indomethacin,excluding the normal control group(which received 1%carboxy methyl cellulose)and the per se group(received 200 mg/kg AH seeds extract).The test group rats were then given 2 doses of AH seeds extract(100 and 200 mg/kg,respectively),while the standard group was given ranitidine(50 mg/kg).On the 11th day,rats in all groups were sacrificed,and their stomach was isolated to calculate the ulcer index,and other parameters such as blood prostaglandin(PGE2),tissue superoxide dismutase(SOD),catalase(CAT),malonyldialdehyde(MDA),and glutathione(GSH).All isolated stomach tissues were analyzed for histopathological findings.Results The phytochemical examination shows that the AH seeds contain alkaloids,flavonoids,saponins,phenolic components,and glycosides.LCMS analysis confirms the presence of quercetin and rutin.The AH seeds extract showed significant improvement in gastric mucosa conditions after indomethacin-induced gastric lesions(P<0.01).Further marked improvement in blood PGE2 and antioxidant enzymes,SOD,CAT,MDA and GSH,were observed compared with self-healing and untreated ulcer-induced groups(P<0.01).Histopathology results confirmed that AH seeds extract improved the mucosal layer and gastric epithelial membrane in treated groups compared to untreated ulcer-induced groups.Conclusions LCMS report confirms the presence of quercetin and rutin in AH seeds ethanolic extract.The therapeutic effect of AH seeds extract against indomethacin-induced ulcer in rat model indicated the regene

关 键 词：Aesculus hippocastanum L. pharmacological action PHYTOCHEMICAL physicochemical analysis liquid chromatography and mass spectroscopy analysis gastro-protective activity 

分 类 号：R285.5[医药卫生—中药学]