2018-2021年广州市环境污水脊髓灰质炎病毒和其他肠道病毒监测  

Environmental surveillance for poliovirus and other enteroviruses in Guangzhou city sewage from 2018 to 2021

作　　者：张薇[1] 李彩霞 曾汉日[1] 柯碧霞[1] 陈美钟 黄淑芬 邓小玲[1] 李柏生[1] 郑焕英[1] Zhang Wei;Li Caixia;Zeng Hanri;Ke Bixia;Chen Meizhong;Huang Shufen;Deng Xiaoling;Li Baisheng;Zheng Huanying(Guangdong Provincial Center for Disease Control and Prevention/Guangdong Workstation for Emerging Infectious Disease Control and Prevention/Guangdong Provincial Key Laboratory of Pathogen Detection for Emerging Infectious Disease Response,Guangzhou 511430,Guangdong,China;School of Public Health,Southern Medical University,Guangzhou 510515,Guangdong,China)

机构地区：[1]广东省疾病预防控制中心,广东新发突发传染病防治工作站,广东省新发传染病应急检测技术研究与应用重点实验室,广东广州511430 [2]南方医科大学公共卫生学院,广东广州510515

出　　处：《中国疫苗和免疫》2023年第4期383-387,共5页Chinese Journal of Vaccines and Immunization

基　　金：广东省医学科学技术研究基金项目(B2022156)。

摘　　要：目的 了解广州市环境污水中脊髓灰质炎病毒(Poliovirus, PV)和非脊髓灰质炎肠道病毒(Non-polio enterovirus, NPEV)现状。方法 选择广州市某污水处理厂于2018-2021年每月采集环境污水样本,进行PV和NPEV分离和血清型鉴定,分析血清型构成。结果 在2018-2021年192份环境污水样本中共分离到299株PV和505株NPEV。在PV分离株中,PV1、PV2、PV3血清型分别为80株(26.76%)、0株(0.00%)、219株(73.24%);1株PV3为疫苗衍生PV(Vaccine-derived poliovirus, VDPV),其他均为疫苗株,其中3株PV3和1株PV1为疫苗高变异PV(Vaccine hypervariable poliovirus, VHPV)。在NPEV分离株中,E11、CVB5和CVB3血清型分别为210株(41.58%)、88株(17.43%)和88株(17.43%)。结论 2018-2021年广州市环境污水中未发现野生型PV和PV2血清型,NPEV优势血清型为E11、CVB5和CVB3。Objective To describe polioviruses(PV)and non-polio enteroviruses(NPEV)found in Guangzhou city sewage.Methods We selected one sewage disposal plant in Guangzhou and collected monthly sewage samples during 2018 to 2021.We isolated and serotyped PV and NPEV strains to determine serotype proportions.Results In total,299 PV strains and 505 NPEV strains were isolated from 192 environmental sewage samples during the study period.Among PVs,80(26.76%),0(0.00%),and 219(73.24%)were PV1,PV2,and PV3 serotypes,respectively;one PV3 was a vaccine-derived poliovirus(VDPV)and the rest were vaccine strains,in which three PV3 and one PV1 were vaccine hypervariable poliovirus(VHPV).Among NPEV strains,210(41.58%),88(17.43%),and 88(17.43%)were E11,CVB5,and CVB3.Conclusions No wild PVs or PV2s were found in environmental sewage of Guangzhou during 2018 to 2021.The predominant NPEV serotypes were E11,CVB5,and CVB3.

关 键 词：肠道病毒 脊髓灰质炎病毒 非脊髓灰质炎肠道病毒 环境污水 监测 

分 类 号：R186[医药卫生—流行病学]