湖北省2018-2022年儿童手足口病肠道病毒病原谱和柯萨奇病毒A6型基因特征  

Pathogenic spectrum of enteroviruses and genetic characteristics of Coxsackievirus A6 in children with hand,foot,and mouth disease from 2018 to 2022 in Hubei province

作　　者：潘锴 周康平 徐军强[1] 蔡昆[1] Pan Kai;Zhou Kangping;Xu Junqiang;Cai Kun(Hubei Provincial Center for Disease Control and Prevention,Wuhan 430079,Hubei,China)

机构地区：[1]湖北省疾病预防控制中心,湖北武汉430079

出　　处：《中国疫苗和免疫》2023年第4期422-426,共5页Chinese Journal of Vaccines and Immunization

基　　金：湖北省自然科学基金项目(2019CFB402和2020CFB516);湖北省卫生健康委员会科研项目(WJ2021M208);国家自然科学基金项目(U21A20423);湖北省公共卫生青年拔尖人才项目。

摘　　要：目的 分析湖北省儿童手足口病(Hand, foot, and mouth disease, HFMD)肠道病毒(Enterovirus, EV)病原谱和柯萨奇病毒A6型(Coxsackievirus A6,CV-A6)基因特征。方法 收集湖北省2018-2022年儿童HFMD病例标本,进行EV核酸检测、分离培养、VP1区全长基因扩增和序列测定,分析EV检测阳性率和CV-A6基因特征。结果 2018-2022年湖北省儿童HFMD病例EV检测总阳性率为65.17%(16 523/25 354),其中EV-A71、CV-A16和其他EV阳性数分别占EV总阳性数的0.98%、29.98%和69.08%,阳性率分别为0.64%、19.54%和45.02%。VP1区全长基因系统进化分析显示,选取的212株CV-A6毒株均处于D3基因亚型D3a进化分支。结论 湖北省2018-2022年儿童HFMD病例EV-A71呈低水平流行,CV-A6属于D3基因亚型且呈优势流行趋势。Objective To analyze the pathogenic spectrum of enteroviruses(EV)and the genetic characteristics of Coxsackievirus A6(CV-A6)in children with hand,foot,and mouth disease(HFMD)in Hubei province.Methods We collected samples from children with HFMD from 2018 to 2022 in Hubei for EV nucleic acid detection,isolation and culture,whole gene amplification,and sequencing of VP1 regions.We analyzed EV positivity rates and genetic characteristics of CV-A6.Results Overall EV positivity was 65.17%(16523/25354)among children with HFMD.EV-A71,CV-A16,and other EVs accounted for 0.98%,29.98%,and 69.08%of all EVs isolated,respectively,with positivity rates of 0.64%,19.54%,and 45.02%.Phylogenetic analysis of whole genes of the VP1 region showed that all 212 CV-A6 strains were located in the D3a branch of D3 subgenotype.Conclusions EV-A71 circulated at a low level;CV-A6 belonged to D3 subgenotype and became a predominant strain in children with HFMD from 2018 to 2022 in Hubei.

关 键 词：手足口病 肠道病毒 柯萨奇病毒A6型 病原谱 基因特征 

分 类 号：R186[医药卫生—流行病学] R512.62[医药卫生—公共卫生与预防医学]