中华跌打丸抗炎镇痛药效学研究及对佐剂关节炎大鼠炎症的影响  

Study on the anti-inflammatory and analgesic pharmacodynamics of Zhonghua Dida Pill and its effect on inflammation in arthritis model rats

作　　者：陈原原 金龙[1] 刘建勋[1] 郝伟[1] 李磊[1] 任均国 付建华[1] 郭浩[1] Chen Yuanyuan;Jin Long;Liu Jianxun;Hao Wei;Li Lei;Ren Junguo;Fu Jianhua;Guo Hao(Beijing Key Laboratory of Pharmacology of Chinese Materia Medica,Institute of Basic Medical Science of Xiyuan Hospital,China Academy of Chinese Medical Sciences,Beijing 100091,China)

机构地区：[1]中国中医科学院西苑医院基础医学研究所、中药药理北京市重点实验室,北京100091

出　　处：《国际中医中药杂志》2023年第8期977-982,共6页International Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(82074060)。

摘　　要：目的考察中华跌打丸抗炎镇痛的药效学作用,探讨其对佐剂关节炎模型大鼠的作用机制。方法采用3种炎症模型和2种疼痛模型考察中华跌打丸的抗炎镇痛作用;建立佐剂关节炎大鼠模型,将大鼠按随机数字表法分为正常组、模型组、地塞米松组(0.8 mg/kg)及中华跌打丸2.0、1.0、0.5 g/kg组,各组连续灌胃相应药物5周,1次/d。分别于给药后1、3、5周测量各组大鼠足趾容积,计算肿胀度;计算大鼠脏器指数,并采用HE染色观察关节软骨组织病理改变;免疫组织化学方法检测关节组织IL-1β、TNF-α、TGF-β表达。结果与对照组比较,中华跌打丸2.0 g/kg组大鼠足跖肿胀度降低(P<0.05),小鼠耳肿胀度降低(P<0.05),大鼠肉芽肿干重减轻(P<0.05);中华跌打丸1.4 g/kg组小鼠扭体次数减少(P<0.05),给药3 h后痛阈值升高(P<0.05);给药3~5周,中华跌打丸2.0 g/kg组大鼠足跖肿胀度降低(P<0.05),胸腺指数降低(P<0.05),关节组织中IL-1β、TNF-α和TGF-β表达降低(P<0.05)。结论中华跌打丸具有明显的抗炎镇痛作用,可通过降低IL-1β、TNF-α、TGF-β等炎症因子水平发挥对佐剂关节炎模型大鼠的治疗作用。Objective To investigate the anti-inflammatory and analgesic effects of Zhonghua Dieda Pills;To preliminarily explore its mechanism on adjuvant arthritis model rats.Methods Three inflammatory models and two pain models were used to investigate the anti-inflammatory and analgesic effects of Zhonghua Dieda Pills.After establishing the adjuvant arthritis rat model,the rats were divided into normal group,model group,dexamethasone group(0.8 mg/kg),and Zhonghua Dieda Pills(2.0,1.0,0.5 g/kg)groups according to random number table method.Each group was given corresponding drugs once a day for 5 weeks.The toe volume was measured at 1,3 and 5 weeks after administration,and the swelling degree was calculated;the organ indices of rats were calculated and the histopathological changes of articular cartilage were observed by HE staining;the expressions of interleukin-1β(IL-1β),tumor necrosis factor-α(TNF-α)and transforming growth factor-β(TGF-β)in joint tissues were detected by immunohistochemistry.Results Zhonghua Dieda Pills(2.0 g/kg)group significantly reduced the swelling of foot and plantar of rats,reduced the swelling of ear of mice,and reduced the dry weight of granuloma of rats(P<0.05);Zhonghua Dieda Pills(1.4 g/kg)group significantly reduced the number of twisting of rats,and the pain threshold after 3 h of administration was significantly higher than that of the control group(P<0.05);Zhonghua Dieda Pills(2.0 g/kg)group significantly reduced the swelling of the foot and metatarsal of arthritic rats after 3-5 weeks of administration(P<0.05),decreased the thymus index(P<0.05),and reduced the expression levels of IL-1β,TNF-α and TGF-β in joint tissues(P<0.05).Conclusion Zhonghua Dieda Pills have confirmed anti-inflammatory and analgesic effects,which may play a therapeutic role in adjuvant arthritis model rats by reducing the levels of inflammatory factors such as IL-1β,TNF-α and TGF-β.

关 键 词：中华跌打丸 关节炎 实验性 抗炎 镇痛 大鼠 

分 类 号：R285.5[医药卫生—中药学]