半夏秫米汤对痰湿内阻慢性失眠模型大鼠5-羟色胺受体及5-羟色胺、5-羟基吲哚乙酸的影响  

Effects of Banxia Shumi Decoction on 5-hydroxytryptamine receptor,5-HT,and 5-HIAA of chronic insomnia rats with internal obstruction of phlegm-dampness

作　　者：张艳 杨嫚 刘西建 张喆 李琳 辛丹 Zhang Yan;Yang Man;Liu Xijian;Zhang Zhe;Li Lin;Xin Dan(College of Traditional Chinese Medicine,Shandong University of Traditional Chinese Medicine,Jinan 250355,China;Yanshan Campus Health Clinic of Shandong Provincial Committee Party School,Jinan 250014,China)

机构地区：[1]山东中医药大学中医学院,济南250355 [2]山东省委党校燕山校区卫生所,济南250014

出　　处：《国际中医中药杂志》2023年第8期983-988,共6页International Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(82174117);山东省自然科学基金重大基础项目(ZR2019ZD23);山东省中医药科技发展计划项目(2019-0034);山东省研究生教育优质课程项目(SDYKC21050);山东中医药大学优秀青年科学基金项目(2018zk44)。

摘　　要：目的观察半夏秫米汤对痰湿内阻慢性失眠模型大鼠5-羟色胺1A受体(5-HT_(1A)R)、5-羟色胺2A受体(5-HT_(2A)R)及5-羟色胺(5-HT)、5-羟基吲哚乙酸(5-HIAA)的影响,探讨该方化痰通利、引阳入阴安神的作用机制。方法将48只Wistar大鼠按随机数字表法分为正常组,模型组,半夏秫米汤低、中、高剂量组,地西泮组,每组8只。除正常组外,其余各组采用“高脂饲料+单平台水环境法”制备痰湿内阻慢性失眠大鼠模型,半夏秫米汤低、中、高剂量组灌胃半夏秫米汤4.69、9.38、18.75 g/kg,地西泮组灌胃地西泮水溶液0.52 mg/kg,1次/d,连续灌胃2周。正常组、模型组灌胃等体积生理盐水。采用qPCR法检测大鼠脑干5-HT_(1A)R、5-HT_(2A)R mRNA水平,采用Western blot法检测大鼠中缝核5-HT_(1A)R、5-HT_(2A)R蛋白表达,采用高效液相色谱-质谱(HPLC-MS)法检测大鼠脑干5-HT、5-HIAA含量。结果与模型组比较,半夏秫米汤低、中、高剂量组及地西泮组5-HT_(1A)R mRNA水平升高(P<0.01);半夏秫米汤高剂量组、地西泮组5-HT_(2A)R mRNA水平降低(P<0.05),5-HT_(1A)R、5-HT_(2A)R蛋白表达升高(P<0.05或P<0.01);半夏秫米汤中、高剂量组及地西泮组5-HT水平升高(P<0.05或P<0.01);半夏秫米汤各剂量组及地西泮组5-HIAA水平升高(P<0.05或P<0.01)。结论半夏秫米汤可能通过调控5-HT_(1A)R、5-HT_(2A)R、5-HT、5-HIAA对痰湿内阻慢性失眠模型大鼠进行干预。Objective To observe the effects of Banxia Shumi Decoction on 5-HT_(1A)R,5-HT_(2A)R,5-HT,and 5-HIAA of chronic insomnia(CI)rats with internal obstruction of phlegm-damp(IOPD)type,to investigate the mechanisms of Banxia Shumi Decoction on resolving and draining dampness,guiding yang into yin and tranquilizing mind.Methods A total of 48 Wistar rats were divided into control group,model group,Banxia Shumi Decoction low-dosage group,medium-dosage group,high-dosage group,and diazepam group according to random number table method,with 8 rats in each group.Except the control group,the CI with IOPD rats model were prepared by the method of"high-fat diet+single-platform water environment"in other groups.The rats in the Banxia Shumi Decoction low-,medium-,high-dosage group were treated with Banxia Shumi Decoction by gavage at the dose of 4.69,9.38 and 18.75 g/kg respectively,the rats in the diazepam group were given 0.52 mg/kg diazepam aqueous solution by gavage,and the rats in the control group and model group were given the equal volume normal saline,once a day for consecutive 2 weeks.The mRNA expressions of 5-HT_(1A)R,5-HT_(2A)R in rat brain stem were detected by qPCR,the protein expressions of 5-HT_(1A)R,5-HT_(2A)R in rat brain raphe nucleus were detected by Western blot,and the contents of 5-HT and 5-HIAA in rat brain stem were determined by HPLC-MS.Results Compared with model group,the expression of 5-HT_(1A)R mRNA significantly increased in the Banxia Shumi Decoction low-,medium-,high-dosage group,and diazepam group(P<0.01);the expression of 5-HT_(2A)R mRNA significantly decreased in the Banxia Shumi Decoction high-dosage group and diazepam group(P<0.05),and the expression of 5-HT_(1A)R and 5-HT_(2A)R significantly increased in the Banxia Shumi Decoction high-dosage group and diazepam group(P<0.05 or P<0.01);5-HT content significantly increased in the Banxia Shumi Decoction medium-,high-dosage group and diazepam group(P<0.05 or P<0.01);5-HIAA content significantly increased in the Banxia Shumi Decoction low-,medium-

关 键 词：失眠症 半夏秫米汤 痰湿内阻 5-羟色胺 5-羟色胺受体 大鼠 

分 类 号：R285.5[医药卫生—中药学]