FSHR基因rs1394205位点多态性与卵巢反应性关系的研究  

Relationship between rs1394205 polymorphism of FSH receptor gene and ovarian response

作　　者：尹倩倩[1,2] 郑建华 曹义娟[1] 严晓南[1] 杨冬梓[2] YIN Qian-qian;ZHENG Jian-hua;CAO Yi-juan;YAN Xiao-nan;YANG Dong-zi(Center for Reproductive Medicine,Xuzhou Central Hospital,Xuzhou 221009;Center for Reproductive Medicine,Sun Yat-sen Memorial Hospital,Sun Yat-Sen University,Guangzhou 510120;Department of Obstetrics and Gynecology,Xuzhou Central Hospital,Xuzhou 221009)

机构地区：[1]徐州市中心医院生殖中心,徐州221009 [2]中山大学附属孙逸仙纪念医院生殖中心,广州510120 [3]徐州市中心医院妇产科,徐州221009

出　　处：《生殖医学杂志》2023年第9期1393-1398,共6页Journal of Reproductive Medicine

基　　金：国家自然科学基金(81571405);江苏省自然科学基金(BK20161169);徐州医科大学附属医院科技发展基金(XYFY202205)。

摘　　要：目的探讨IVF周期中患者卵泡刺激素受体(FSHR)基因rs1394205位点多态性与卵巢反应性的关系。方法回顾性分析2019年3月至2022年6月在徐州市中心医院生殖中心第一次接受IVF-ET且年龄在35岁以下的327例不孕症患者,提取外周血基因组DNA并进行rs1394205位点测序。根据rs1394205位点等位基因的不同,将患者分为GA型(n=156)、AA型(n=79)和GG型(n=92),比较各基因型患者卵巢反应性的差异。结果FSHR基因rs1394205位点的G、A等位基因频率分别是51.99%和48.01%。GG、GA及AA 3种基因型的频率分别为28.13%、47.71%和24.16%。GG型患者的促性腺激素(Gn)启动量及总量较GA型和AA型患者呈减少的趋势(P=0.070;P=0.129),但差异尚无统计学意义(P>0.05);AA型患者卵巢低反应的发生率(11.37%)较GA型(3.21%)和GG型(2.17%)患者显著增高(P<0.05)。3组患者的HCG日雌二醇(E 2)水平、HCG日直径≥14 mm卵泡数、获卵数、可利用胚胎数及临床妊娠率均无显著差异(P>0.05)。二元Logistic回归分析结果显示体质量指数(BMI)、抗苗勒管激素(AMH)水平、Gn总量及rs1394205位点AA基因型是卵巢低反应性的独立影响因素(P<0.05)。结论FSHR基因rs1394205位点的AA基因型是IVF患者卵巢低反应发生的独立影响因素。Objective:To explore the relationship between rs1394205 polymorphisms of FSH receptor(FSHR)gene and ovarian response in patients with IVF treatment.Methods:The clinical data of 327 women aged under 35 years old who underwent the first IVF-ET cycle at the Reproductive Center of Xuzhou Central Hospital from March 2019 to June 2022 were retrospectively analyzed.Genomic DNAs of peripheral white blood cells were extracted and sequencing rs1394205 locus was performed.The patients were divided into GA type(n=156),AA type(n=79),and GG type(n=92),and the differences in ovarian responsiveness were compared among the different genotypes of patients.Results:The allele frequencies of rs1394205 were 51.99%for G and 48.01%for A.Genotype frequency was 28.13%for GG,47.71%for GA and 24.16%for AA.The gonadotrophin(Gn)initiation dose and the total Gn doses were decreased in GG type patients compared with GA and AA type patients(P=0.070;P=0.129),but the difference was not significant.The incidence of ovarian poor response in AA type patients(11.37%)was significantly higher than that in GA type(3.21%)and GG type(2.17%)patients(P<0.05).There were no significant differences in the estradiol(E 2)level on HCG day,the number of follicles with diameter≥14 mm on HCG day,the number of oocytes retrieved,the number of available embryos and the clinical pregnancy rate among the three groups(P>0.05).Binary logistic regression analysis showed that body mass index(BMI),anti-Mullerian hormone(AMH),total Gn doses and genotype AA at rs1394205 were independent influencing factors of ovarian poor response(P<0.05).Conclusions:Genotype AA of rs1394205 locus of FSHR gene was an independent influencing factor in the occurrence of ovarian poor response in patients with IVF treatment.

关 键 词：卵泡刺激素受体 基因多态性 卵巢低反应 体外受精-胚胎移植 

分 类 号：R711.6[医药卫生—妇产科学]