基于SSR标记的河南省假俭草群体遗传多样性研究  

A study of genetic diversity in centipedegrass populations in Henan based on SSR markers

作　　者：吉轶楠 任雪锋 苟甜甜 臧国长[1] 郑轶琦[1] JI Yi-nan;REN Xue-feng;GOU Tian-tian;ZANG Guo-zhang;ZHENG Yi-qi(College of Horticulture and Plant Protection,Henan University of Science and Technology,Luoyang 471000,China)

机构地区：[1]河南科技大学园艺与植物保护学院,河南洛阳471000

出　　处：《草业学报》2023年第9期198-212,共15页Acta Prataculturae Sinica

基　　金：河南省自然科学基金项目(18230041001)资助。

摘　　要：基于SSR分子标记研究河南省假俭草群体遗传多样性,结果表明所用引物共检测到99个等位基因,引物多态性信息含量为0.53~0.88,多态性条带百分率为98.75%,所选引物的多态性较高。分子方差分析(AMOVA)表明河南假俭草居群的遗传分化主要发生于居群内(86.96%),居群间存在较频繁的基因交流。聚类及Mantel检验结果表明地理距离与遗传距离间无相关性。群体遗传结构分析显示,141份假俭草材料分为3个亚群,与主坐标分析(PCoA)结果一致。冗余分析(RDA)结果表明最干旱月份降水量是影响河南省假俭草种群结构的主要因素。结合FDIST2和BayeScan两种方法共同检测到4个异常位点,异常位点检出比例为4.04%,环境关联分析检测到部分与环境因子关联的位点,今后可进一步开展转录组学研究,进行基因功能注释,系统地揭示假俭草的适应性进化机制。Based on SSR molecular markers,this study analyzed the genetic diversity of centipedegrass populations in Henan.Ninety nine alleles were detected with the primers,the polymorphic information content of the primers was 0.53-0.88,and the percentage of polymorphic bands was 98.75%,showing that the selected primers had high polymorphism.Analysis of molecular variance(AMOVA)showed that genetic differentiation of centipedegrass in Henan occurred mainly within populations(86.96%)and showed frequent gene exchange among populations.Cluster analysis and a Mantel test showed that there was no correlation between geographic and genetic distance.The analysis of population genetic structure showed that 141 populations evaluated were divided into 3 subgroups,which was consistent with the results of principal coordinate analysis(PCoA).Redundancy analysis(RDA)showed that precipitation in the driest month was the main factor affecting the population structure of centipedegrass in Henan.Four outlier loci were detected by combining FDIST2 and BayeScan,and the detection rate of outlier loci was 4.04%.Some loci associated with environmental factors were detected by environmental correlation analysis.In future,we can carry out further transcriptomics research,annotate gene functions,and systematically reveal the adaptive evolutionary mechanism of centipedegrass.

关 键 词：假俭草 遗传分化 遗传多样性 群体遗传结构 

分 类 号：S688.4[农业科学—观赏园艺]