主流条件下间歇曝气抑制NOB与微生物动态响应  

The way of coupling intermittent aeration strategy to inhibit NOB activity in mainstream environment and the dynamic response of microorganisms

作　　者：谢怡漪 李祥[1,2,3] 袁怡 许佩玲 黄勇 吴鹏[1,2] XIE Yi-yi;LI Xiang;YUAN Yi;XU Pei-ling;HUANG Yong;WU Peng(School of Environmental Science and Engineering,Suzhou University of Science and Technology,Suzhou 215009,China;National and Local Joint Engineering Laboratory for Municipal Sewage Resource Utilization Technology,Suzhou University of Science and Technology,Suzhou 215009,China;Jiangsu Collaborative Innovation Center of Technology and Material of Water Treatment,Suzhou University of Science and Technology,Suzhou 215009,China)

机构地区：[1]苏州科技大学环境科学与工程学院,江苏苏州215009 [2]城市污水资源化利用技术国家地方联合工程实验室,江苏苏州215009 [3]江苏水处理技术与材料协同创新中心,江苏苏州215009

出　　处：《中国环境科学》2023年第9期4616-4625,共10页China Environmental Science

基　　金：国家自然科学基金资助项目(51938010);苏州市科技计划项目-民生科技项目(SS202025);江苏省水处理技术与材料协同创新中心(XTCXSZ2022-1);江苏省环境科学与工程重点实验室(JSHJZDSYS-202004)。

摘　　要：在主流条件下,采用不同的接种泥源和间歇曝气过程的停曝比,分析了实现稳定亚硝化过程的关键条件和硝化过程抑制机理.结果表明,仅依靠低溶解氧(DO)和间歇曝气,亚硝化体系难以获得NO_(2)-N的积累.当耦合厌氧氨氧化工艺后,在保证同等氨氮转化的环境下,出水NO_(3)-N浓度由41.64mg/L降至11.54mg/L,硝化过程获得显著抑制.定量分析结果表明,NOB在低DO耦合间歇曝气环境中适应性较强,运行15d后最大NOB拷贝数增加2.9倍,而在含有厌氧氨氧化菌的混合系统中,最大NOB拷贝数仅增加1.4倍.微生物群落结构变化结果表明,在低DO耦合间歇曝气环境中,接种混合污泥系统中的NOB,其丰度上升速度较硝化污泥系统中的快.因此,本研究揭示强化NO_(2)-N在好氧阶段的利用,降低NO_(2)-N暴露浓度是间歇曝气过程中抑制NOB的关键条件.The key conditions and inhibition mechanism in nitrification process were analysed by using different sludge sources and anoxic/aerobic ratios of intermittent aeration process under mainstream conditions.The results showed that nitrite accumulation was difficult to obtain in partial nitrification system relying only under low-dissolve oxygen(DO)and intermittent aeration.When coupled with anammox process,NO_(3)-N concentration in effluent decreased from 41.64mg/L to 11.54mg/L under the same NH_(4)^(+)-N conversion environment,and the nitrification process was significantly inhibited.The quantitative analysis shows that Nitrate-oxidizing bacteria(NOB)had strong adaptability in the environment of low-DO and intermittent aeration,with a maximum increase of 2.9 times after 15days of operation,while in a mixed system with anammox bacteria,the maximum increase was only 1.4 times.The results of microbial community structure change showed that in low DO coupled intermittent aeration environment,the abundance of NOB inoculated in mixed sludge system increased faster than that in nitrification sludge system.Therefore,strengthening nitrite utilization and reducing its exposure concentration in aerobic stage is a key condition for achieving NOB inhibition in intermittent aeration process.

关 键 词：部分亚硝化 间歇曝气 抑制机制 接种泥源 停曝比 

分 类 号：X703[环境科学与工程—环境工程]