创伤性肌筋膜触发点的形成对大鼠骨骼肌肌梭内NT-3及受体TrkC的表达影响  

Effect of the Formation of Traumatic Myofascial Trigger Point on the Expression of NT-3 and Receptor TrkC in Skeletal Muscle Spindle of Rats

作　　者：刘琳[1] 刘庆广 黄强民 LIU Lin;LIU Qingguang;HUANG Qiangmin(Department of Rehabilitation,Nanjing Sport Institute,Nanjing 210014,China;International College of Football,Tongji University,Shanghai 200092,China;Shanghai Yiyang TCM Clinic,Shanghai 201822,China;School of Kinesiology,Shang‐hai University of Sport,Shanghai 200438,China)

机构地区：[1]南京体育学院运动健康学院,江苏南京210014 [2]同济大学国际足球学院,上海200092 [3]上海逸养中医门诊部,上海201822 [4]上海体育学院运动健康学院,上海200438

出　　处：《南京体育学院学报》2023年第7期44-49,共6页Journal of Nanjing Sports Institute

基　　金：国家自然科学基金面上项目(81470105);国家自然科学基金青年科学基金(32000829);江苏省高校“青蓝工程”项目。

摘　　要：目的:确定创伤性肌筋膜触发点的形成对其周围肌梭组织内部蛋白和基因表达水平的影响,并分析触发点牵涉痛临床特点和针刺“打跳”的分子生物学依据。方法:32只7周龄雄性SD大鼠随机均分为实验组和对照组,实验组采取对腓肠肌定点钝性打击结合离心运动的模式进行连续8 w造模。造模结束后两组均正常饲养4w。12 w结束后,检测造模成功指标,并在此基础上提取各组腓肠肌触发点组织和非触发点组织,以及各组比目鱼肌、胫骨前肌和跖肌处骨骼肌分别进行NT-3和NT-3的受体TrkC蛋白的免疫印迹学实验和基因的聚合酶链式反应实验。结果:与对照组相比,NT-3蛋白在触发点大鼠内外侧腓肠肌中的含量均表现为降低(P<0.05),而NT-3 mRNA在触发点大鼠内外侧腓肠肌、趾肌、胫骨前肌和比目鱼肌中的含量均表现为降低(P<0.05);TrkC蛋白在触发点大鼠内侧腓肠肌、趾肌和胫骨前肌中的含量也均表现为降低(P<0.05),而TrkC mRNA在触发点大鼠内外侧腓肠肌和趾肌含量也均表现为降低(P<0.05)。结论:慢性肌筋膜触发点的形成诱发了受累肌和多块关联肌肌梭内NT-3、受体TrkC蛋白和基因的下调。Objective:To investigate the effect of traumatic myofascial trigger points(MTrPs)on the expression of protein and gene in the surrounding muscle spindle tissue,and to analyze the molecular biological basis of referred pain and“needling jump”of MTrPs.Methods:Thirty-two male SD rats(7 weeks old)were randomly divided into experimental group and control group.Rat model of chronic MTrPs was established by striking on fascia muscles and eccentric exercise,once per week,for continuous 8 weeks.Then those rats were reared normally and took rest for 4 weeks.At the end of the 12th week,those rats were examined for locating MTrPs with palpable taut band,the local twitch response and spontaneous electrical activity.Subsequently,the MTrPs muscles of gastrocnemius and non-MTrPs muscles of soleus,tibialis anterior and metatarsal muscles in experimental groups were extracted to detect the expression of protein and gene of NT-3 and TrkC by using Western blot and from polymerase chain reac‐tion(PCR)tests,and compared with those in the conventional control group.Results:Compared with non-MTrPs rats,the con‐tents of NT-3 protein in the gastrocnemius muscle of MTrPs rats decreased significantly(P<0.05),and the contents of NT-3 recep‐tor TrkC protein in the medial gastrocnemius,muscles and tibialis anterior muscle of MTrPs rats also decreased significantly(P<0.05);the contents of NT-3 mRNA in the medial and lateral gastrocnemius,toe muscles,tibialis anterior and soleus muscles of MTrPs rats decreased significantly(P<0.05),and the contents of TrkC mRNA in the medial and lateral gastrocnemius and toe muscles of MTrPs rats were also decreased significantly(P<0.05).Conclusion:the formation of chronic MTrPs can induce signifi‐cant down-regulate expression of proteins and genes of NT-3 and TrkC at MTrPs and some related muscles.

关 键 词：肌梭 肌筋膜触发点 NT-3 TRKC 牵涉痛 

分 类 号：G812[文化科学—体育学]