LY294002对过敏性鼻炎-哮喘综合征大鼠氧自由基、TRPV1神经元敏感性及TSLP表达的影响  

Effects of LY294002 on oxygen free radical,TRPV1 neuron sensitivity and TSLP expression in allergic rhinitis-asthma syndrome rats

作　　者：李丽 许翀 柴若楠 LI Li;XU Chong;CHAI Ruonan(Department of Allergy,General Hospital of PLA Northern Theater Command,Shenyang 110016,China;Department of Allergy,Jinzhou Medical University,Jinzhou 121001,Liaoning,China)

机构地区：[1]中国人民解放军北部战区总医院过敏反应科,辽宁沈阳110016 [2]锦州医科大学过敏反应科,辽宁锦州121001

出　　处：《西部医学》2023年第9期1276-1281,共6页Medical Journal of West China

基　　金：辽宁省自然基金资助项目(2019-MS-356)。

摘　　要：目的研究LY294002对过敏性鼻炎-哮喘综合征(CARAS)大鼠氧自由基、TRPV1神经元敏感性及TSLP表达的影响。方法将40只大鼠随机分为健康组(健康大鼠常规饲养)、模型组(卵蛋白致敏和鼻部滴注攻击法建立过敏性鼻炎-哮喘综合征大鼠模型)、治疗组(模型+静脉注射LY2940020.5 mg/kg)、对照组(模型+布地奈德气雾剂)。ELISA与免疫组织化学法检测TSLP表达,黄嘌呤氧化酶法检测SOD,TBA法检测MDA,HE染色观察大鼠肺组织病理形态学变化,免疫荧光检测TRPV1表达,免疫印迹检测NF-кB、IкB蛋白表达。结果与健康组相比,模型组大鼠支气管及周围产生大量炎性细胞,支气管黏膜水肿、增厚,管腔狭窄,黏液分泌增多;与模型组比较,治疗组与对照组肺组织病理明显有所改善。与健康组相比,模型组TRPV1、MDA、TSLP、NF-кB升高,SOD、IкB降低(P<0.05);与模型组相比,治疗组与对照组TRPV1、MDA、TSLP、NF-кB降低,SOD、IкB升高(P<0.05);治疗组与对照组各指标相比无差异(P>0.05)。结论通过抑制TRPV1、TSLP、MDA并促进SOD表达,降低了CARAS大鼠气道神经源性炎症、防止气道高反应性的产生并改善了氧化应激损伤及病理水平,从而发挥治疗作用。Objective To study the effects of LY294002 on oxygen free radical,TRPV1 neuronal sensitivity and TSLP expression in allergic rhinitis-asthma syndrome rats.Methods Forty rats were randomly divided into healthy group(healthy rats were routinely fed),model group(model of allergic rhinitis asthma syndrome was established by ovin sensitization and nasal drip attack),treatment group(model+intravenous injection of LY2940020.5 mg/kg),and control group(model+budesonide aerosol).The expression of TSLP was detected by ELISA and immunohistochemistry,SOD was detected by xanthine oxidase,MDA was detected by TBA,histopathological changes of lung tissues were observed by HE staining,the expression of TRPV1 was detected by immunofluorescence,and the expressions of NF-кB and IкB were detected by western blot.Results Compared with the healthy group,there were a lot of inflammatory cells infiltration,bronchial mucosa edema and thickening,lumen narrowing and mucus secretion increased in model group.Compared with model group,the pathological changes of lung tissue in treatment group and control group were significantly improved.Compared with the healthy group,TRPV1,MDA,TSLP and NF-кB were increased,while SOD and IкB were decreased in the model group(P<0.05).Compared with the model group,TRPV1,MDA,TSLP and NF-кB were decreased in the treatment group and the control group,while SOD and IкB were increased(P<0.05).There was no difference in the indexes between the treatment group and the model group(P>0.05).Conclusion By inhibiting TRPV1,TSLP and MDA and promoting the expression of SOD,the neurogenic inflammation of airway in CARAS rats is reduced,the generation of airway hyperreactivity is prevented and the level of oxidative stress injury and pathology is improved,thus playing a therapeutic role.

关 键 词：LY294002 过敏性鼻炎-哮喘综合征大鼠 氧自由基 TRPV1、TSLP 

分 类 号：R765.21[医药卫生—耳鼻咽喉科]