浊度仪在液基细胞学数字化定量制片中的应用探讨  

Discussion on the application of turbidimeter in liquid-based cytologic digital quantitative production

作　　者：李碧奇 何思倩 杨丽[1] 史琳 梁悦 韦康来[1] LI Bi-qi;HE Si-qian;YANG Li;SHI Lin;LIANG Yue;WEI Kang-lai(The Second Affiliated Hospital of Guangxi Medical University,Nanning,Guangxi 530000,China;不详)

机构地区：[1]广西医科大学附属第二医院,广西南宁530000 [2]广西医大睿谷医学检验有限公司

出　　处：《中国卫生检验杂志》2023年第15期1799-1801,共3页Chinese Journal of Health Laboratory Technology

基　　金：广西自然科学基金项目(2022GXNSFAA035496);自治区卫生健康委自筹经费科研课题(Z-A20230620)。

摘　　要：目的研究使用麦氏浊度仪对液基细胞学制片进行数字标准定量转化,检验是否可以标准化制片细胞数。方法选取细胞液基样本360份,分别设立批内实验组、批内对照组、批间实验组、批间对照组、室间实验组、室间对照组,每组60份,实验组使用麦氏浊度仪进行定量液基制片,对照组按传统方式进行液基制片。结果目的细胞量精密度分析批内实验组-批间对照组差异有统计学意义(P<0.05),四分位数间距批内实验组<批内对照组;批内实验组-批间实验组差异无统计学意义(P>0.05),四分位数间距批间实验组<批间对照组;批内实验组-室间实验组差异无统计学意义(P>0.05),批内对照组-室间对照组差异有统计学意义(P<0.05);覆盖率实验组3组均为0.0%,对照组总覆盖率分别为10.0%、13.3%和3.3%。结论基于麦氏浊度仪对细胞悬液进行数字化调整可以实现液基标准化定量制片,提高实验的精密度和减少细胞重叠情况。Objective This paper aims to study the digital standard quantitative transformation of liquid-based cytological sections by McDonald turbidimeter,and to test whether the number of cells can be standardized.Methods A total of 360 cases of cell liquid-based samples were selected,and an intra-batch experimental group,an intra-batch control group,an inter-batch experimental group,an inter-batch control group,an inter-compartment experimental group,and an inter-compartment control group were set up respectively,with 60 cases in each group.The experimental group was used for liquid-based quantitative production by McDonald turbidimeter,while the control group was used for liquid-based production by traditional means.Results There was statistical significance on the difference in cell quantity precision between intra-batch experimental group and inter-batch control group(P<0.05).Interquartile spacing shows intra-batch experimental group was less than intra-batch control group,and there was no statistical significance on the difference between the intra-experimental group and inter-experimental group(P>0.05).The interquartile spacing shows inter-experimental group was less than inter-group control,and there was no statistical significance on the difference between the intra-batch experimental group and the inter-laboratory control group(P>0.05),but there was statistical significance on the difference between the intra-batch control group and the inter-laboratory control group(P<0.05).The coverage of the experimental group was 0.0%,and the total coverage of the control group was 10.0%,13.3% and 3.3%,respectively.Conclusion The digital adjustment of cell suspension based on McDonald turbidimeter can achieve the quantitative preparation of liquid base standardization,improve the precision of the experiment and reduce the overlap of cells.

关 键 词：液基细胞学制片 浊度仪 数字化定量制片 目的细胞量 精密度 人工智能诊断 

分 类 号：R446[医药卫生—诊断学]