室温条件下电场缓解高氮负荷抑制厌氧氨氧化性能的研究  

The ability of electric field to relieve the inhibition of high nitrogen loading on Anammox at room temperature

作　　者：张驰[1] 王超 沙宏举 胡筱敏[2] ZHANG Chi;WANG Chao;SHA Hongju;HU Xiaomin(School of Municipal&Environmental Engineering,Shenyang Jianzhu University,Shenyang Liaoning 110168;School of Resources&Civil Engineering,Northeastern University,Shenyang Liaoning 110819)

机构地区：[1]沈阳建筑大学市政与环境工程学院,辽宁沈阳110168 [2]东北大学资源与土木工程学院,辽宁沈阳110819

出　　处：《环境污染与防治》2023年第9期1202-1207,共6页Environmental Pollution & Control

基　　金：国家科技重大专项(No.2012ZX07505002)。

摘　　要：以全混合厌氧反应器(CSTR)构建厌氧氨氧化(Anammox)系统,利用脉冲电源提供电场,考察室温条件下高氮负荷对Anammox脱氮效果的长期影响;通过酶标仪分析Anammox细菌中关键酶活性,阐释电场对Anammox细菌活性的影响。结果表明,电场能够强化Anammox细菌关键酶活性和脱氮效果,在11.38 kg/(d·m^(3))的进水氮负荷下仍能及时转化氮元素,增强脱氮效果,总氮去除率可以维持在85%以上。电场能够提升Anammox细菌活性,提高新陈代谢速率,降低高氮负荷对微生物细胞的毒性抑制作用,但过高氮负荷(15.93 kg/(d·m^(3)))的抑制作用将超过电场的促进作用,使亚硝酸盐等毒性物质累积,破坏细胞活性,导致Anammox脱氮效果降低甚至崩溃。The continuous stirred tank reactor(CSTR)was used to construct anaerobic ammonia oxidation(Anammox)system and pulse power supply was used to provide electric field to investigate the long-term effect of high nitrogen loading on denitrification performance of Anammox process under room temperature condition.The key enzyme activities of Anammox bacteria were analyzed by microplate reader to explain the effect of electric field on the activity of Anammox bacteria.The results showed that the suitable electric field could enhance the key enzyme activity and nitrogen removal effect of Anammox process.Even under the nitrogen loading condition of 11.38 kg/(d·m^(3)),Anammox could also transform nitrogen efficiently and enhance the nitrogen removal,with the TN removal rate remained above 85%.The suitable electric field could enhance the Anammox bacteria activity,increase the metabolism rate and reduce the inhibition of high nitrogen loading.However,the inhibitory effect of excessive nitrogen load(15.93 kg/(d·m^(3)))exceeded the promoting effect of electric field,so that nitrite and other toxic substances would accumulate and destroy the cell activity and reduce the nitrogen removal effect,even causing the Anammox process broken.

关 键 词：室温 高氮负荷 厌氧氨氧化 电场 微生物活性 

分 类 号：X703[环境科学与工程—环境工程]