烟嘧磺隆高效降解复合菌系的构建及降解特性  

Construction and degradation characteristics of high efficient degradation complex microbial system of nicosulfuron

作　　者：白文斌 郝佳丽[1] 曹昌林 张建华[1] BAI Wenbin;HAO Jiali;CAO Changlin;ZHANG Jianhua(Sorghum Research Institute,Shanxi Agricultural University,Shanxi Key Laboratory of Sorghum Genetic and Germplasm Innovation,Jinzhong 030600,Shanxi,China)

机构地区：[1]山西农业大学高粱研究所,高粱遗传与种质创新山西省重点实验室,山西晋中030600

出　　处：《农药》2023年第9期669-674,共6页Agrochemicals

基　　金：国家重点研发计划项目子课题(2021YFD1901103-6);山西省现代农业产业技术体系建设(杂粮)(2023-03);2023年度“特”“优”农业高质量发展科技支撑工程项目(TYGC23-56);酿造专用高粱育种及利用山西省科技创新团队(201805D131012-6);国家基础性长期性监测项目(ZX04S0406000,AES2020S0406)。

摘　　要：[目的]构建烟嘧磺隆高效降解复合菌系并明确其降解特性,为高效修复烟嘧磺隆污染土壤提供理论支撑。[方法]通过富集驯化培养,从山西省不同生态区烟嘧磺隆污染土壤中筛选出5株烟嘧磺隆降解菌,通过16S rDNA和ITS序列分析鉴定降解菌的分类地位。通过全组合构建高效降解复合菌修复体系,并通过单因素试验明确其降解特性。[结果]筛选获得10株具有烟嘧磺隆降解能力的菌株,其中5株菌株降解能力较强。经16S rDNA和ITS序列鉴定和系统发育分析,5株烟嘧磺隆降解菌株分别为A枯草芽孢杆菌、B黑曲霉、C草酸青霉、D土曲霉和E绿木霉。全组合复配结果表明,由3种菌株组成的复合菌系对烟嘧磺隆降解率最好,其中ABD组合对烟嘧磺隆降解能力最高,较单株菌降解率最高的菌株D降解率提高23.74%;将筛选的A、B、D进行不同比例复配,菌株最佳复配比A∶B∶D为2∶3∶1时,烟嘧磺隆降解率最高达98.31%,各菌株对烟嘧磺隆降解的影响效果A>B>D。复合菌系较单一菌株增加了适宜的温度、pH值和烟嘧磺隆初始浓度范围,最适培养降解条件为接种量2%~5%,温度30~40℃,pH 7.0,烟嘧磺隆初始质量浓度50~200 mg/L。[结论]筛选获得的复合菌系ABD在烟嘧磺隆残留污染土壤的微生物修复应用中具有潜在应用前景。[Aims]To construct the complex microbial system of high efficient degradation of nicosulfuron and clarify its degradation characteristics,and provide theoretical support for efficient remediation of nicosulfuron contaminated soil.[Methods]Five strains of nitrosulfuron-degrading bacteria were screened from the soil polluted by nicosulfuron in different ecological regions of Shanxi Province through enrichment and acclimation culture,and their taxonomic status was identified by 16S rDNA and ITS sequence analysis.An efficient composite bacterial repair system was constructed through the whole combination,and its degradation characteristics were determined by single factor test.[Results]Ten strains with the degradation ability of nicosulfuron were screened,and 5 of them had strong degradation ability.According to 16S rDNAand ITS sequence identification and phylogenetic analysis,the 5 strainswere Bacillus subtilis(A),Aspergillus niger(B),Penicillium oxalate(C),A.aspergillus(D)and Trichoderma viridis(E)respectively.The results of all combinations showed that the degradation rate of the complex microbial system composed of 3 strains was the best,and the degradation rate of ABD combination was the highest,which was 23.74%higher than that of single strain.When the selected strains A,B and D were mixed,the optimal ratio of A:B:D was 2:3:1,and the degradation rate of nicosulfuron was up to 98.31%.The effect of each strain on nicosulfuron degradation was A>B>D.Compared with the single strain,the optimum temperature,pH and substrate concentration range were increased.The optimum conditions for the degradation of the composite strain were:inoculation dosage of 2%-5%,temperature of 30-40℃,pH 7.0,and mass concentration of nicosulfuron 50-200 mg/L.[Conclusions]The composite strain ABD screened in this study has a potential application in the remediation of soil contaminated by icosulfuron residue.

关 键 词：烟嘧磺隆降解菌 鉴定 复配 降解特性 

分 类 号：TQ450.2[化学工程—农药化工]