望春花(M.biondi)花提取物缓解UVB导致皮肤损伤的作用研究  

The study of Magnolia biondii flower extract in relieving UVB-induced skin damage

作　　者：黄芳 张营 熊玥 周利丹 卢伊娜 Fang Huang;Ying Zhang;Yue Xiong;Lidan Zhou;Yina Lu(Shanghai JAKA Biotech Co.,Ltd.,Shanghai 200241,China)

机构地区：[1]上海珈凯生物科技有限公司,上海200241

出　　处：《日用化学工业（中英文）》2023年第9期1065-1072,共8页China Surfactant Detergent & Cosmetics

摘　　要：富含木兰脂素的望春花(M.biondi)花提取物(MB)被用来研究其缓解紫外线导致的皮肤损伤作用。在中波紫外线(UVB)诱导的人永生化表皮细胞(HaCaT)和以UVB诱导的HaCaT细胞培养上清液作为刺激物诱导的血管内皮细胞(HUVEC)上,通过免疫荧光染色、酶联免疫吸附法检测细胞损伤下的不同关键指标的变化。结果显示,HaCaT细胞经UVB照射后16~72 h,环丁烷嘧啶二聚体(CPD)产生量显著升高,高迁移率族蛋白B1(HMGB1)表达上升,且逐步从细胞核迁移至细胞质,热休克家族蛋白27(Hsp27)表达量也上升,同时白介素6(IL-6)、白介素8(IL-8)、前列腺素E2(PGE-2)的表达量显著增加。而HaCaT细胞培养上清液刺激HUVEC细胞24 h后,内皮素-1(ET-1)、细胞间粘附分子-1(ICAM-1)表达量均显著升高。0.1%~0.5%的MB同步处理下,可显著抑制HaCaT细胞中CPD的产生量、HMGB1的表达量,减少HMGB1的核转移,促进Hsp27的表达,降低细胞因子的表达量,同时可显著减少HUVEC细胞中的ET-1,ICAM-1表达量。因此,MB可通过降低DNA损伤,减少HMGB1的合成与核转移,减少细胞因子表达量,降低血管通透性,来缓解皮肤受到紫外线照射后的损伤作用。Magnolia biondii flower extract(MB),which isrich in magnoliol was used to study its efficacy on alleviating UVB-induced skin damage.Immunofluorescence staining and enzyme-linked immunosorbent assay were applied to detect different key biomarkers on UVB-induced keratinocytes(HaCaT)and vascular endothelial cells(HUVEC),which induced by HaCaT cell culture supernatant after UVB stimulated for 48 h.The results show that the levels of cyclobutane pyrimidine dimer(CPD)and high mobility group box protein B1(HMGB1)increase significantly in HaCaT cells from 16 h to 72 h after UVB irradiation,and also HMGB1 is gradually migrated from the nucleus to the cytoplasm.The expression of heat shock family protein 27(Hsp27)also increases,as well as the cytokines such as interleukin 6(IL-6),interleukin 8(IL-8),and prostaglandin E2(PGE-2).Meanwhile,the expression levels of endothelin-1(ET-1)and intercellular adhesion molecule-1(ICAM-1)are significantly increased after HUVEC cells stimulated by cell culture supernatant for 24 h.Under the synchronous treatment of 0.1%-0.5%MB,the levels of CPD and HMGB1 in HaCaT cells are significantly inhibited,with an increase expression of Hsp27,and a decreased of the nuclear transfer of HMGB1 and cytokines.In HUVEC cells,the expression levels of ET-1and ICAM-1 are also significantly inhibited after MB treatment.Therefore,MB can alleviate the damage of skin after UV irradiation by reducing DNA damage,reducing the synthesis and nuclear transfer of HMGB1,and inhibiting the expression of cytokines and vascular permeability.

关 键 词：望春花(M.biondi)花提取物 紫外线 HMGB1 ICAM-1 皮肤损伤 

分 类 号：TQ658[化学工程—精细化工]