Expansion and adaptive evolution of the WRKY transcription factor family in Avicennia mangrove trees  

作　　者：Xiao Feng Guohong Li Weihong Wu Haomin Lyu Jiexin Wang Cong Liu Cairong Zhong Suhua Shi Ziwen He 

机构地区：[1]State Key Laboratory of Biocontrol,Guangdong Key Laboratory of Plant Resources,School of Life Sciences,Southern Marine Science and Engineering Guangdong Laboratory(Zhuhai),Sun Yat-sen University,Guangzhou 510275,China [2]Greater Bay Area Institute of Precision Medicine(Guangzhou),Fudan University,Guangzhou 511458,China [3]Hainan Academy of Forestry(Hainan Academy of Mangrove),Haikou 571100,China

出　　处：《Marine Life Science & Technology》2023年第2期155-168,共14页海洋生命科学与技术（英文）

基　　金：We thank Qipian Chen,Zuyao Liu and Min Liu for technical support.We thank the editors and reviewers for their helpful contributions.The project was supported by the National Natural Science Foundation of China(32170230,31971540 and 31830005);the Guangdong Basic and Applied Basic Research Foundation(2023B1515020083);the Innovation Group Project of Southern Marine Science and Engineering Guangdong Laboratory(Zhuhai)(311021006).

摘　　要：Mangroves are adapted to intertidal zones,which present extreme environmental conditions.WRKYs are among the most prominent transcription factors(TFs)in higher plants and act through various interconnected networks to regulate responses to multiple abiotic stressors.Here,based on omic data,we investigated the landscape and evolutionary patterns of WRKYs in the main mangrove genus Avicennia.We found that both the number and the proportion of TFs and WRKYs in Avicennia species exceeded their inland relatives,indicating a signifcant expansion of WRKYs in Avicennia.We identifed 109 WRKY genes in the representative species Avicennia marina.Comparative genomic analysis showed that two recent wholegenome duplication(WGD)events played a critical role in the expansion of WRKYs,and 88%of Avicennia marina WRKYs(AmWRKYs)have been retained following these WGDs.Applying comparative transcriptomics on roots under experimental salt gradients,we inferred that there is high divergence in the expression of WGD-retained AmWRKYs.Moreover,we found that the expression of 16 AmWRKYs was stable between freshwater and moderately saline water but increased when the trees were exposed to high salinity.In particular,14 duplicates were retained following the two recent WGD events,indicating potential neo-and sub-functionalization.We also found that WRKYs could interact with other upregulated genes involved in signalling pathways and natural antioxidant biosynthesis to enhance salt tolerance,contributing to the adaptation to intertidal zones.Our omic data of the WRKY family in A.marina broadens the understanding of how a TF family relates to the adaptive evolution of mangroves.

关 键 词：ADAPTATION AVICENNIA MANGROVE Transcription factor Whole-genome duplication WRKY 

分 类 号：Q94[生物学—植物学]