Genomic diversity and ecological distribution of marine Pseudoalteromonas phages  

作　　者：Kaiyang Zheng Yue Dong Yantao Liang Yundan Liu Xinran Zhang Wenjing Zhang Ziyue Wang Hongbing Shao Yeong Yik Sung Wen Jye Mok Li Lian Wong Andrew McMinn Min Wang 

机构地区：[1]College of Marine Life Sciences,Institute of Evolution and Marine Biodiversity,and Frontiers Science Center for Deep Ocean Multispheres and Earth System,Ocean University of China,Qingdao 266100,China [2]UMT-OUC Joint Center for Marine Studies,Qingdao 266003,China [3]Institute of Marine Biotechnology,Universiti Malaysia Terengganu(UMT),21030 Kuala Nerus,Malaysia [4]Institute for Marine and Antarctic Studies,University of Tasmania,Hobart,Australia [5]Haide College,Ocean University of China,Qingdao 266100,China [6]The Afliated Hospital of Qingdao University,Qingdao 266000,China

出　　处：《Marine Life Science & Technology》2023年第2期271-285,共15页海洋生命科学与技术（英文）

基　　金：This work was supported by the Laoshan Laboratory(No.LSKJ202203201);National Key Research and Development Program of China(2022YFC2807500);Natural Science Foundation of China(No.41976117,42120104006,42176111 and 42188102);and the Fundamental Research Funds for the Central Universities(202172002,201812002,202072001 and Andrew McMinn).

摘　　要：Pseudoalteromonas,with a ubiquitous distribution,is one of the most abundant marine bacterial genera.It is especially abundant in the deep sea and polar seas,where it has been found to have a broad metabolic capacity and unique co-existence strategies with other organisms.However,only a few Pseudoalteromonas phages have so far been isolated and investigated and their genomic diversity and distribution patterns are still unclear.Here,the genomes,taxonomic features and distribution patterns of Pseudoalteromonas phages are systematically analyzed,based on the microbial and viral genomes and metagenome datasets.A total of 143 complete or nearly complete Pseudoalteromonas-associated phage genomes(PSAPGs)were identifed,including 34 Pseudoalteromonas phage isolates,24 proviruses,and 85 Pseudoalteromonas-associated uncultured viral genomes(UViGs);these were assigned to 47 viral clusters at the genus level.Many integrated proviruses(n=24)and flamentous phages were detected(n=32),suggesting the prevalence of viral lysogenic life cycle in Pseudoalteromonas.PSAPGs encoded 66 types of 249 potential auxiliary metabolic genes(AMGs)relating to peptidases and nucleotide metabolism.They may also participate in marine biogeochemical cycles through the manipulation of the metabolism of their hosts,especially in the phosphorus and sulfur cycles.Siphoviral and flamentous PSAPGs were the predominant viral lineages found in polar areas,while some myoviral and siphoviral PSAPGs encoding transposase were more abundant in the deep sea.This study has expanded our understanding of the taxonomy,phylogenetic and ecological scope of marine Pseudoalteromonas phages and deepens our knowledge of viral impacts on Pseudoalteromonas.It will provide a baseline for the study of interactions between phages and Pseudoalteromonas in the ocean.

关 键 词：PSEUDOALTEROMONAS BACTERIOPHAGES GENOME Classifcation ECOLOGY 

分 类 号：Q178.53[生物学—水生生物学]