An improved blood hemorrhaging treatment using diatoms frustules,by alternating Ca and light levels in cultures  

作　　者：Qinfeng Li Zheng He HusseinERozan Chao Feng Xiaojie Cheng Xiguang Chen 

机构地区：[1]College of Marine Life Science,Sanya Oceanographic Institution,Ocean University of China,Qingdao 266003,China [2]Laoshan Laboratory,Qingdao 266237,China [3]Department of Biochemistry,Faculty of Agriculture,Al-Azhar University,Cairo,Egypt

出　　处：《Marine Life Science & Technology》2023年第3期316-325,共10页海洋生命科学与技术（英文）

基　　金：The work was supported by National Natural Science Foundation of China(U22A20588,82172095);Shandong Provincial Natural Science Foundation(ZR2019QD005);Qingdao Science and Technology Demonstration and Guidance Project(20-3-4-50-nsh).

摘　　要：Hemorrhage control requires hemostatic materials that are both efective and biocompatible.Among these,diatom biosilica(DBs)could signifcantly improve hemorrhage control,but it induces hemolysis(the hemolysis rate>5%).Thus,the purpose of this study was to explore the infuence of Ca^(2+)biomineralization on DBs for developing fast hemostatic materials with a low hemolysis rate.Here,CaCl_(2)was added to the diatom medium under high light(cool white,fuorescent lamps,67.5µmol m^(−2) s^(−1)),producing Ca-DBs-3 with a particle size of 40-50μm and a Ca^(2+)content of Ca-DBs-3 obtained from the higher concentration CaCl_(2)group(6.7 mmol L^(−1))of 0.16%.The liquid absorption capacity of Ca-DBs-3 was 30.43±0.57 times its dry weight;the in vitro clotting time was comparable to QuikClot®zeolite;the hemostatic time and blood loss using the rat tail amputation model were 36.40±2.52 s and 0.39±0.12 g,which were 40.72%and 19.50%of QuikClot®zeolite,respectively.Ca-DBs-3 showed no apparent toxicity to L929 cells(cell viability>80%)and was non-hemolysis(the hemolysis rate<2%).This study prepared Ca-DBs-3 with a rapid hemostatic efect and good biocompatibility,providing a path to develop diatom biosilica hemostatic materials.

关 键 词：Hemorrhage control Diatom biosilica Ca2+biomineralization Hemolysis rate Hemostatic efect 

分 类 号：R31[医药卫生—基础医学]