麝香黄芪复方滴丸含药血清促进骨髓间充质干细胞增殖分化  

Shexiang Huangqi compound dripping pills-containing serum promotes proliferation and differentiation of bone marrow mesenchymal stem cells

作　　者：陈娜[1,2] 王燕琳[3] 孙慧芳[3] 樊飞燕 李东红 张运克[1,2] Chen Na;Wang Yanlin;Sun Huifang;Fan Feiyan;Li Donghong;Zhang Yunke(First Affiliated Hospital of Henan University of Chinese Medicine,Zhengzhou 450046,Henan Province,China;Henan University of Chinese Medicine,Zhengzhou 450046,Henan Province,China;First Affiliated Hospital of Zhengzhou University,Zhengzhou 450046,Henan Province,China)

机构地区：[1]河南中医药大学第一附属医院,河南省郑州市450046 [2]河南中医药大学,河南省郑州市450046 [3]郑州大学第一附属医院,河南省郑州市450046

出　　处：《中国组织工程研究》2024年第19期2960-2966,共7页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金项目(81974564),项目负责人:张运克;中原科技创新领军人才(224200510027),项目负责人:张运克。

摘　　要：背景:骨髓间充质干细胞(bone marrow mesenchymal stem cells,BMSCs)已被广泛用于治疗神经系统疾病,但由于血脑屏障的限制以及干细胞在受损部位存活率、分化率低,导致治疗效果有限。目的:探讨麝香黄芪复方滴丸含药血清对BMSCs增殖、迁移和向星形胶质细胞分化的影响。方法:SD雄性大鼠连续灌胃麝香黄芪复方滴丸5 d后进行腹主动脉取血,分离血清备用。采用CCK-8法检测体积分数5%,10%,20%含药血清对BMSCs增殖的影响;划痕实验观察体积分数10%含药血清对BMSCs横向迁移的影响;Transwell小室培养BMSCs,用结晶紫染色和DAPI核染色观察体积分数10%含药血清对BMSCs纵向迁移的影响;用含体积分数10%含药血清的诱导液或与星形胶质细胞共培养观察BMSCs向星形胶质细胞分化情况。结果与结论:①体积分数10%和20%含药血清在第2,3天促进细胞增殖更明显,且两种体积分数无统计学差异;②在划痕30,48 h时,10%含药血清组BMSCs迁移量显著高于对照组;③10%含药血清组BMSCs穿过Transwell小室滤过膜的数量高于对照组;④体积分数10%含药血清可能促进BMSCs向星形胶质细胞方向分化,但分化作用较弱,星形胶质细胞能进一步促进含药血清诱导BMSCs向星形胶质细胞方向分化;⑤结果表明,体积分数10%含药血清能促进BMSCs体外增殖、迁移;与星形胶质细胞共培养可能促进BMSCs向星形胶质细胞方向分化。BACKGROUND:Bone marrow mesenchymal stem cells have been widely used to treat neurological diseases.However,due to limitations of the blood-brain barrier,low survival rate and differentiation rate of stem cells at damaged sites,the therapeutic effect is limited.OBJECTIVE:To investigate the effects of Shexiang Huangqi compound dripping pills on proliferation,migration and astrocyte differentiation of bone marrow mesenchymal stem cells.METHODS:Male SD rats were treated with Shexiang Huangqi compound dripping pills for 5 days after continuous gavage.Blood was collected from the abdominal aorta and serum was separated for later use.The effect of 5%,10%and 20%drug-containing serum on the proliferation of bone marrow mesenchymal stem cells was detected by CCK-8 assay.The effect of 10%drug-containing serum on lateral migration of bone marrow mesenchymal stem cells was observed by scratch test.Bone marrow mesenchymal stem cells were cultured in Transwell cells.The effects of 10%drug-containing serum on longitudinal migration of bone marrow mesenchymal stem cells were observed by crystal violet staining and DAPI nuclear staining.Differentiation of bone marrow mesenchymal stem cells into astrocytes was observed by inducing solution with 10%drug-containing serum or co-culture with astrocytes.RESULTS AND CONCLUSION:(1)10%and 20%drug-containing serum promoted cell proliferation more significantly on days 2 and 3,and there was no statistical difference between the two concentrations.(2)At 30 and 48 hours,bone marrow mesenchymal stem cell migration in 10%drug-containing serum group was significantly higher than that in the control group.(3)The number of bone marrow mesenchymal stem cells filtered through Transwell cells in 10%drug-containing serum group was higher than that in the control group.(4)10%drug-containing serum might promote the differentiation of bone marrow mesenchymal stem cells to astrocytes,but the differentiation effect was weak,and astrocytes might further promote the differentiation of bone marrow mesenchymal

关 键 词：缺血性脑卒中 麝香黄芪复方滴丸 含药血清 骨髓间充质干细胞 星形胶质细胞 增殖 迁移 分化 

分 类 号：R459.9[医药卫生—治疗学] R394.2[医药卫生—临床医学] R318