Notch信号通路调控间充质干细胞的增殖与分化  

Notch signaling pathway regulates proliferation and differentiation of mesenchymal stem cells

作　　者：王雪淞 周林 李林材 邹征伟 唐兴坤 卢文明 陈文杰 汪月 叶俊松 Wang Xuesong;Zhou Lin;Li Lincai;Zou Zhengwei;Tang Xingkun;Lu Wenming;Chen Wenjie;Wang Yue;Ye Junsong(Subcenter for Stem Cell Clinical Translation,First Affiliated Hospital of Gannan Medical University,Ganzhou 341000,Jiangxi Province,China;School of Rehabilitation Medicine,Gannan Medical University,Ganzhou 341000,Jiangxi Province,China;Ganzhou Key Laboratory of Stem Cell and Regenerative Medicine,Ganzhou 341000,Jiangxi Province,China;Key Laboratory of Medical Tissue Engineering Materials and Biological Manufacturing of Jiangxi Province,Gannan Medical University,Ganzhou 341000,Jiangxi Province,China;Key Laboratory of Prevention and Treatment of Cardiovascular and Cerebrovascular Diseases,Ministry of Education,Gannan Medical University,Ganzhou 341000,Jiangxi Province,China;College of Nursing,Gannan Medical University,Ganzhou 341000,Jiangxi Province,China)

机构地区：[1]赣南医学院第一附属医院干细胞临床转化分中心,江西省赣州市341000 [2]赣南医学院康复学院,江西省赣州市341000 [3]赣州市干细胞与再生医学重点实验室,江西省赣州市341000 [4]赣南医学院江西省医用组织工程材料与生物制造重点实验室,江西省赣州市341000 [5]赣南医学院心脑血管疾病防治教育部重点实验室,江西省赣州市341000 [6]赣南医学院护理学院,江西省赣州市341000

出　　处：《中国组织工程研究》2024年第19期3076-3083,共8页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金项目(32060232),项目负责人:叶俊松;江西省自然科学基金面上项目(20212BAB206057),项目负责人:叶俊松;江西省卫生健康委科技计划项目资助(20191079),项目负责人:叶俊松。

摘　　要：背景:研究发现,Notch的配体和受体都是细胞膜表面蛋白,是介导细胞间通讯的一种重要蛋白,Notch信号通路在间充质干细胞增殖与分化过程中起着至关重要的调控作用。目的:就Notch信号通路对间充质干细胞增殖与分化过程的调节机制进行综述,总结和阐述Notch信号通路如何调控间充质干细胞的增殖与分化相关研究进展,为未来利用干细胞治疗各种相关疾病提供理论支持。方法:由第一作者应用计算机在中国知网、万方、维普、PubMed、Web of Science和Nature数据库检索涉及Notch信号通路调控间充质干细胞增殖与分化的相关文献,中文检索词为“间充质干细胞,Notch,Notch信号通路,增殖,分化”,英文检索词为“mesenchymal stem cells,MSC,Notch,Notch signaling pathway,proliferation,differentiation”,结合文献追溯法查找部分文献,最终纳入87篇文献进入综述分析。结果与结论:①Notch信号通路是一条存在于多细胞生物中保守的信号通路,通过受体与配体结合介导相邻细胞间的通信,在调节细胞分化、增殖、凋亡和细胞周期中发挥重要作用。②间充质干细胞是一类具有自我增殖和多向分化潜能的成体干细胞,可受外界信号通路的调控从而影响其增殖与分化,而Notch信号通路作为其中之一,当Notch配体被激活后,Notch蛋白会经历2次蛋白水解裂解,释放出Notch细胞内结构域NICD随后进入细胞核,进而促进靶基因的转录以达到调控骨髓、脂肪和脐带等不同来源的间充质干细胞增殖与分化的作用,但是调控同物种不同组织来源间充质干细胞在增殖与分化过程中的具体机制有所不同。③Notch信号通路可以调控间充质干细胞分化成不同的靶细胞,但是由于分化的靶细胞不同,Notch信号通路的受体或配体表达水平有差异。④临床上以Notch信号通路为靶点,促进间充质干细胞治疗各种难治性疾病,如再生障碍性贫血症、严BACKGROUND:It was found that the ligands and receptors of Notch are both cell membrane surface proteins,which are important proteins to mediate intercellular communication,and the Notch signaling pathway plays a crucial regulatory role in the proliferation and differentiation of mesenchymal stem cells.OBJECTIVE:To review the regulatory mechanism of the Notch signaling pathway on the proliferation and differentiation of mesenchymal stem cells,summarize and clarify the research advance in how the Notch signaling pathway regulates the proliferation and differentiation of mesenchymal stem cells,and provide theoretical support for the future use of stem cells to treat various related diseases.METHODS:By using the computer,the first author searched the relevant studies involving Notch signaling pathway regulation of mesenchymal stem cell proliferation and differentiation on CNKI,Wanfang,VIP,PubMed,Web of Science,and Nature databases with Chinese search terms“mesenchymal stem cells,Notch,Notch signaling pathway,proliferation,differentiation”and the English search terms“mesenchymal stem cells,MSC,Notch,Notch signaling pathway,proliferation,differentiation”.Part of the literature was searched in combination with the literature tracing method.Finally,87 articles were included in the review analysis.RESULTS AND CONCLUSION:(1)Notch signaling pathway is a conserved signaling pathway in multicellular organisms,which plays an important role in regulating cell differentiation,proliferation,apoptosis,and the cell cycle by mediating communication between neighboring cells through receptor-ligand binding.(2)Mesenchymal stem cells are a class of adult stem cells with self-proliferative and multi-directional differentiation potential,which can be regulated by external signaling pathways to affect their proliferation and differentiation.Notch signaling pathway,as one of them,when Notch ligands are activated,the Notch proteins will undergo two protein hydrolysis cleavages to release Notch intracellular structural domain NICD,wh

关 键 词：NOTCH信号通路 信号通路 干细胞 间充质干细胞 临床意义 细胞命运 增殖 分化 综述 

分 类 号：R459.9[医药卫生—治疗学] R363[医药卫生—临床医学] R364