网络药理学探讨雾水葛防治Ⅱ型糖尿病的物质基础及作用机制  

Exploration of the Subtance Basis and Action Mechanism of Mistletoe Kudzu Against Type Ⅱ Diabetes Mellitus With Network Pharmacology

作　　者：李汉成 曾茗 莫紫娇 马德运 LI Hancheng;ZENG Ming;MO Zijiao;MA Deyun(School of Food and Pharmaceutical Engineering,Zhaoqing University,Zhaoqing,Guangdong 526061,China)

机构地区：[1]肇庆学院食品与制药工程学院,广东肇庆526061

出　　处：《肇庆学院学报》2023年第5期47-53,共7页Journal of Zhaoqing University

基　　金：广东省重点领域研发计划基金资助项目(2021B0707010008);广东省科技厅农村科技特派员课题资助项目(KTP20200176);肇庆市科技计划基金资助项目(2022040314001);肇庆学院博士科研启动基金资助项目(220003);肇庆学院科研基金资助项目(QN202224);肇庆学院大学生创新创业训练计划资助项目(S202310580075)。

摘　　要：通过网络药理学探讨雾水葛防治Ⅱ型糖尿病的物质基础及作用机制.首先通过文献调研获取雾水葛的活性成分,Pubchem等数据库分析雾水葛主要成分核心靶点,运用GeneCards获取Ⅱ型糖尿病靶点,通过微生信软件筛选雾水葛-Ⅱ型糖尿病的靶点交集,进一步构建“活性成分-靶点-疾病”网络和蛋白质相互作用(PPI)网络,并进行KEGG和GO分析.研究发现雾水葛中含有丰富的熊果酸、东莨菪素、槲皮素及芹菜素等,这些活性成分通过调控INSR、ERBB2、AKT1及TEK等重要靶点,进一步影响PI3K/Akt通路、HIF-1通路及5-羟色胺能突触等信号通路发挥防治Ⅱ型糖尿病的作用.Network pharmacology was used to investigate the substance basis and mechanism of action of Mistletoe Kudzu in the prevention and treatment of type Ⅱ diabetes.Firstly,we obtained the active ingredients of mugwort through literature research,analyzed the core targets of the main ingredients of mugwort in Pubchem and other databases,used GeneCards to obtain the targets of type Ⅱ diabetes,screened the target intersection of mugwort and type Ⅱ diabetes by Microbiology software,and further constructed the"active ingredient-target-disease"network and protein-protein interaction(PPI).The network and the protein-protein interaction(PPI)network were further constructed,and KEGG and GO analyses were performed.These active ingredients were found to be rich in ursolic acid,scopolamine,quercetin and apigenin,which further affect the PI3K/Akt pathway,HIF-1 pathway and 5-hydroxytryptophan synapse by regulating the important targets such as INSR,ERBB2,AKT1 and TEK to prevent and treat type Ⅱ diabetes.

关 键 词：网络药理学 雾水葛 Ⅱ型糖尿病 物质基础 作用机制 

分 类 号：R961.1[医药卫生—药理学]