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作 者:任小冬 龚志海 饶伟强 雷胜桥 刘力军 李秋明 REN Xiaodong;GONG Zhihai;RAO Weiqiang;LEI Shengqiao;LIU Lijun;LI Qiuming(Procincial Nature Management Service of South China Tigers in Northern Guangdong,Shaoguan,Guangdong 512023,China;Hangzhou Aidikang Medical Laboratory Center Co.,Ltd.Hangzhou,Zhejiang310030,China)
机构地区:[1]广东粤北华南虎省级自然保护区管理处,广东韶关512023 [2]杭州艾迪康医学检验中心有限公司,浙江杭州310030
出 处:《林业与环境科学》2023年第4期27-33,共7页Forestry and Environmental Science
基 金:韶关市科技计划项目(200811214533884)。
摘 要:为保护华南虎Panthera tigris amoyensis物种,以静脉血淋巴细胞G显带和R显带染色技术研究和分析华南虎染色体带型。结果显示,华南虎每条染色体具有独特的带型特征,常染色体中带数最多的为24条带,带数最少的为6条带;性染色体中X染色体为12条带,Y染色体是相对长度最短也是带数最少的染色体,仅3条带。实验成功制备华南虎染色体G带和R带标本,得到248条带的华南虎染色体分带模式,经初步对比发现华南虎染色体G带模式虽与家猫染色体分带高度近似,但也存在少部分明显区别,研究华南虎染色体带型有利于虎及其它猫科动物的遗传学和基因多样性方面研究。In order to protect South China tiger species,the chromosome banding patterns of the South China tiger were studied by G banding and R banding of lymphocytes from venous blood.The results showed that each chromosome of the South China tiger had unique banding characteristics,with 24 bands having the highest number of bands in the autosomes and 6 bands having the lowest number of bands;Among the sex chromosome,the X chromosome has 12 bands,and the Y chromosome has the shortest relative length and the smallest number of bands,with only 3 bands.The experiment successfully prepared G-band and R-band specimens of South China tiger chromosomes and obtained 248 bands of South China tiger chromosome banding patterns.After preliminary comparison,it was found that although the G-band patterns of South China tiger chromosomes were highly similar to those of domestic cats,there were also a few obvious differences.The study of South China tiger chromosome banding patterns is beneficial to the genetic and genetic diversity research of tigers and other feline animals.
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