常见水稻病虫害胁迫对转cry1C基因抗虫水稻Bt蛋白表达量的影响  

Bt protein expression of transgenic cry1C rice under the stress of common rice pests and diseases

作　　者：王欢[1,2] 曹征鸿[2,3] 叶恭银 党聪[2] WANG Huan;CAO Zhenghong;YE Gongyin;DANG Cong(Shanghai Vocational College of Agriculture and Forestry,Shanghai 201699,China;State Key Laboratory of Rice Biology/Ministry of Agricultural and Rural Affairs Key Laboratory of Molecular Biology of Crop Pathogens and Insects/Key Laboratory of Biology of Crop Pathogens and Insects of Zhejiang Province,Institute of Insect Sciences,Zhejiang University,Hangzhou,Zhejiang 310058,China;Forestry Bureau of Yingtan,Yingtan,Jiangxi 335001,China)

机构地区：[1]上海农林职业技术学院,上海201699 [2]浙江大学昆虫科学研究所,水稻生物学国家重点实验室/农业农村部作物病虫分子生物学重点实验室/浙江省作物病虫生物学重点实验室,浙江杭州310058 [3]鹰潭市林业局,江西鹰潭335001

出　　处：《生物安全学报》2023年第3期250-254,共5页Journal of biosafety

基　　金：上海市自然科学基金(19ZR1436500);转基因生物新品种培育重大专项(2016ZX08011-01)。

摘　　要：【目的】明确转Bt基因抗虫水稻在病虫害胁迫下对Bt蛋白表达量的影响。【方法】以Bt水稻T1C-19(表达Cry1C蛋白)为研究对象,探究褐飞虱、白叶枯病等6种水稻常见病虫害胁迫下其体内Bt蛋白的表达量变化。【结果】褐飞虱取食引起T1C-19水稻叶片中Bt蛋白含量降低,而黑尾叶蝉取食显著降低了T1C-19水稻叶鞘中的Bt蛋白含量,白叶枯病侵染导致Bt水稻叶鞘中的Bt蛋白含量显著上升。二化螟、水稻普通矮缩病、稻瘟病的胁迫对转基因水稻T1C-19叶片和叶鞘中的Bt蛋白含量均无显著影响。【结论】病虫害胁迫因种类不同对Bt水稻中Bt蛋白表达量的影响有所不同。这将为Bt水稻的抗虫效果评价提供数据基础,同时为Bt水稻病虫害综合治理提供科学依据。【Aim】The effects of rice pest and disease stress on the Bt protein expression in insect resistant transgenic Bt(Bacillus thuringiensis)rice need to be defined.【Method】In this study,one Bt rice line,T1C-19(expressing Cry1C protein),was used to investigate the changes of Bt protein expression in Bt rice under the stress of six common rice pests and diseases,such as brown planthopper,bacterial blight,and so on.【Result】The results showed that the feeding of brown planthopper caused a decrease in Cry1C protein content in T1C-19 rice leaves,while the feeding of green rice leafhopper significantly reduced Cry1C protein content in T1C-19 rice sheaths,and bacterial blight infestation caused a significant increase in Cry1C protein content in T1C-19 rice sheaths.The stresses of rice stem borer,rice dwarf virus and rice blast had no significant effects on the Bt protein content in both leaves and sheaths in transgenic rice T1C-19.【Conclusion】The Bt protein expression in Bt rice varied from the stress of different pest and disease species.These results will provide a data basis for the evaluation of the insect resistance effect of Bt rice,and also provide some scientific basis for the integrated pest and disease management on Bt rice.

关 键 词：水稻 病虫害 胁迫 BT蛋白 表达量 

分 类 号：S435.11[农业科学—农业昆虫与害虫防治]