嗜菌异小杆线虫H06品系对红棕象甲致病力的时间效应及对血淋巴的影响  

Virulence-time correlation and effects to haemolymph of Heterorhabditis bacteriophora H06 strain to Rhynchophorus ferrugineus

作　　者：吕朝军[1] 张楚毓 钟宝珠[1] 赵建超 陈拓 LU Chaojun;ZHANG Chuyu;ZHONG Baozhu;ZHAO Jianchao;CHEN Tuo(Coconut Research Institute,Chinese Academy of Tropical Agricultural Sciences,Wenchang,Hainan 571339,China)

机构地区：[1]中国热带农业科学院椰子研究所,海南文昌571339

出　　处：《生物安全学报》2023年第3期263-269,共7页Journal of biosafety

基　　金：海南省自然科学基金高层次人才项目(320RC740)。

摘　　要：【目的】阐明嗜菌异小杆线虫H06品系处理对红棕象甲血淋巴免疫系统的影响。【方法】采用室内生物测定和生理生化测定法,研究了嗜菌异小杆线虫H06品系对红棕象甲不同虫态的致病力及对血淋巴各项指标的调节作用。【结果】嗜菌异小杆线虫H06品系对红棕象甲不同虫态的致病力与处理浓度和处理时间均呈正相关性,经嗜菌异小杆线虫H06品系处理后,红棕象甲血淋巴总量和血淋巴酯酶均呈前期升高、后期降低的趋势,处理后72 h的血淋巴总量和酯酶活性与对照相比分别降低39.73%和53.36%;血淋巴蛋白含量与对照相比呈下降的趋势,虽在60 h时有一个短暂的回升,之后又迅速下降,其中在处理后72 h,血淋巴的蛋白含量与对照相比下降74.96%。【结论】嗜菌异小杆线虫H06品系的侵入破坏了红棕象的血淋巴免疫系统,本研究结果可为揭示昆虫病原线虫对红棕象甲的致病机理提供依据。【Aim】Elucidate the effect of Heterorhabditis bacteriophora H06 strain treatment on the hemolymph immune system of Rhynchophorus ferrugineus.【Method】The pathogenicity of the H.bacteriophora H06 strain on R.ferrugineus at different stages and its regulation of various indexes of hemolymph were studied through laboratory bioassay and physio-biochemical determination methods.【Result】The virulence of the H.bacteriophora H06 strain showed a positive correlation with treatment concentration and treatment time on different stages of R.ferrugineus.After treatment with the H.bacteriophora H06 strain,the total hemolymph and hemolymph esterase levels of R.ferrugineus increased in the early stage and decreased later.The total hemolymph content and esterase activity decreased by 39.73% and 53.36%,respectively,72 h after treatment compared with the control.The hemolymph protein content decreased,with a short increase at 60 h,and then decreased rapidly.The hemolymph protein content decreased by 74.96% 72 h after treatment.【Conclusion】The infestation of the H.bacteriophora H06 strain destroyed the hemolymph immune system of R.ferrugineus.The results may provide a theoretical basis for revealing the pathogenic mechanism of the entomopathogenic nematode to R.ferrugineus.

关 键 词：嗜菌异小杆线虫H06品系 红棕象甲 血淋巴 致病机理 

分 类 号：S763.306.4[农业科学—森林保护学]