黏质沙雷氏菌AHPC29对松树萎蔫病的防治效果及其增菌条件  

Control effect of Serratia marcescens AHPC29 on pinewood wiltdisease and optimization of culture conditions for the bacteria

作　　者：黄晨颖 张艳芬 吴守欣 赵莉蔺[2,3] 石娟 HUANG Chenying;ZHANG Yanfen;WU Shouxin;ZHAO Lilin;SHI Juan(Sino-France Joint Laboratory for Invasive Forest Pests in Eurasia,Beijing Forestry University,Beijing 100083,China;State Key Laboratory of Integrated Management of Pest Insects and Rodents,Institute of Zoology,Chinese Academy of Sciences,Beijing 100101,China;CAS Center for Excellence in Biotic Interactions,University of Chinese Academy of Sciences,Beijing 100049,China;Forest Pest Control and Quarantine Station of Lushi County,Sanmenxia,Henan 472200,China)

机构地区：[1]北京林业大学,中法欧亚森林入侵生物联合实验室,北京100083 [2]中国科学院动物研究所,农业虫害鼠害综合治理研究国家重点实验室,北京100101 [3]中国科学院大学,中国科学院生物互作卓越创新中心,北京100049 [4]河南省卢氏县森林病虫害防治检疫站,河南三门峡472200

出　　处：《生物安全学报》2023年第3期270-276,共7页Journal of biosafety

基　　金：国家自然科学基金面上项目(NSFC31970466);国家重点研发计划(2019YFC1200504)。

摘　　要：【目的】从松材线虫的媒介天牛蛹室及其气管中获得的黏质沙雷氏菌Serratia marcescens AHPC29对松材线虫具有致病能力,本研究旨在探究黏质沙雷氏菌AHPC29对松材线虫引起的松树萎蔫病的防治效果以及该菌株在实验室的增菌条件。【方法】通过对温室内人工感染松材线虫的松树灌溉菌剂,分析黏质沙雷氏菌AHPC29对松树萎蔫病的作用效果;通过单组分筛选和正交实验确定培养基组分和培养条件对其生长的影响,探究黏质沙雷氏菌AHPC29的最佳增菌条件。【结果】对于感染松材线虫的松树,灌溉黏质沙雷氏菌AHPC29菌液的处理组生长状态优于对照组,并且树内松材线虫含量显著降低;黏质沙雷氏菌AHPC29增菌的最佳培养基配比为0.1%乳糖、0.5%复合氨基酸、0.5%KNO_(3)、1.5%MgSO_(4),其中影响最大的组分为氮源;培养时其菌液最佳接种量为7%,最佳装液量为40%,最佳转速为180 r·min^(-1),最佳温度为30℃,最适培养时长为36 h。【结论】本研究获得了黏质沙雷氏菌AHPC29的最佳增菌条件,并证实该菌具有良好的松树萎蔫病防治应用潜力,为其作为生防菌的应用提供了理论基础。【Aim】Serratia marcescens AHPC29,which was isolated from the pupa chamber of Monochamus alternatus,a vector of the pinewood nematode,has been shown to be pathogenic to the pinewood nematode.The pinewood nematode,Bursaphelenchus xylophilus,is an important invasive forest pest that can cause pine wilt disease.This study was carried out to investigate the effect of S.marcescens AHPC29 on pine wilt disease under conditions of increasing strain in a laboratory setting.【Method】The effects of S.marcescens AHPC29 on pine wilt disease were analyzed by irrigating bacteria cultures into pine trees that had been artificially infected with pinewood nematodes in a greenhouse.The effects of culture medium components and culture conditions on the growth of S.marcescens AHPC29 were determined by single-component screening and orthogonal experiments to explore the optimal conditions for the growth of S.marcescens AHPC29.【Result】For the pines infected with pinewood nematodes,the growth status of the treatment group irrigated with the AHPC29 strain was better than that of the control group,and the pinewood nematode content in the trees was significantly reduced.The optimal medium composition was 0.1% lactose,0.5% complex amino acids,0.5%KNO_(3),and 1.5%MgSO_(4),among which the most influential component was the nitrogen source.The optimal inoculation ratio was 7%,the optimal filling ratio was 40%,the optimal rotate speed was 180 r·min^(-1),the optimal temperature was 30℃,and the optimal culture time was 36 h.【Conclusion】In this study,the optimal conditions for the growth of S.marcescens AHPC29 were obtained,and it was confirmed that the strain had good application potential in the control of pine wilt disease,which provided a theoretical basis for its application as a biocontrol bacterium.

关 键 词：生防菌 黏质沙雷氏菌 松材线虫 培养条件优化 

分 类 号：S763[农业科学—森林保护学]