毕节地区6个绵羊品种FecB基因微卫星座位多态性研究  

Study on Microsatellite Polymorphism of FecB Gene of Six Sheep Breeds in Bijie

作　　者：宋德荣 吴瑛 林如涛[2] 单春兰 马金萍 孙忠鑫 熊艳玲 刘勇庆 胡飞飞 SONG Derong;WU Ying;LIN Rutao;SHAN Chunlan;MA Jinping;SUN Zhongxin;XIONG Yanling;LIU Yongqing;HU Feifei(Bijie Animal Husbandry and Veterinary Research Institute,Bijie Guizhou 550017,China;College of Animal Science,Guizhou University,Guiyang 550025,China)

机构地区：[1]毕节市畜牧兽医科学研究所,贵州毕节551700 [2]贵州大学动物科学学院,贵阳550025

出　　处：《西南大学学报（自然科学版）》2023年第9期76-84,共9页Journal of Southwest University(Natural Science Edition)

基　　金：贵州省科技支撑计划项目(黔科合支撑[2019]2277号);贵州省草食家畜高效养殖人才基地项目(黔人领发[2018]3号);贵州省科研机构创新能力建设专项(黔科合服企[2021]12号);毕节市科学技术“揭榜挂帅”项目(毕科合重大专项字[2021]2号);财政部和农业农村部国家绒毛用羊产业技术体系项目(CARS-39).

摘　　要：FecB(Fecundity Booroola)是羊繁殖性状的主效基因,该文旨在探讨贵州毕节地区6个绵羊品种FecB基因微卫星座位多态性及与湖羊和威宁绵羊杂交品系繁殖性状的相关性.首先采集6个品种绵羊血浆,提取基因组DNA,选择位于FecB基因中的6个微卫星座位,设计引物,运用聚合酶链式反应扩增微卫星基因序列,以聚丙烯酰胺凝胶电泳银染技术施以微卫星分型.比较各组之间FecB微卫星基因型与等位基因分布,并进行多态性分析.结果显示:6对微卫星引物均能在6个羊群组中稳定扩增.微卫星座位LSCV043,BMS2508,300U,GC101,Bulge5和471U均能检测到3~6个等位基因.其中等位基因114 bp在微卫星座位LSCV043中频率最高;等位基因162 bp在微卫星BMS2508中频率最高;等位基因164 bp在微卫星座位300U中频率最高;等位基因200 bp在微卫星座位GC101中频率;等位基因136 bp在微卫星座位Bulge5中频率最高;等位基因200 bp在微卫星座位471U中频率最高.多态信息含量(PIC)结果显示:300U,GC101两个微卫星座位在各组羊群中均属于高度多态水平(PIC>0.5),而471U座位在各组羊群中均为中度和低度多态基因座.微卫星座位300U在湖羊与威宁绵羊杂交品系中等位基因数为5个,其中基因型148/160 bp对应的最小二乘平均值最高,达到了1.81只;微卫星座位GC101基因型200/238 bp对应的最小二乘平均值最高,达到了1.74只,可用于高繁绵羊的初步辅助选择.FecB is a negative regulator of muscle development.The purpose of this study was to investigate the microsatellite polymorphism of FecB gene in six sheep breeds in Bijie,Guizhou,and its correlation with reproductive traits of Hu sheep and Weining sheep hybrids group.Firstly,plasma of six breeds of sheep was collected,genomic DNA was extracted,six microsatellite sites located in FecB gene were selected,primers were designed,microsatellite sequences were amplified by polymerase chain reaction,and microsatellite typing was performed by polyacrylamide gel electrophoresis and silver staining technology.The distribution of FecB microsatellite genotype and allele among groups was compared,and the polymorphism was analyzed.The results showed that 6 pairs of microsatellite primers could be stably amplified in 6 sheep groups.A total of 3-6 alleles were detected from LSCV043,BMS2508,300U,GC101,Bulge5 and 471U microsatellites.The frequency of 114 bp allele was the highest among LSCV043 microsatellites.The frequency of 162 bp allele was the highest in microsatellite BMS2508.The frequency of 164 bp allele was the highest in microsatellite 300U.The frequency of 200 bp allele was the highest in microsatellite GC101.The 136 bp allele has the highest frequency in the microsatellite Bulge5.The frequency of allele 200 bp was the highest in microsatellite 471U.The results of polymorphism information content(PIC)showed that 300U and GC101 microsatellite loci were highly polymorphic(PIC>0.5)in each group of sheep,while 471U loci were moderate and low polymorphic loci in each group of sheep.The number of alleles of microsatellite loci 300U in Hu sheep and Weining sheep hybrid line is 5,among which the least squares mean value corresponding to genotype 148/160 bp is the highest,reaching 1.81.The least-squares mean corresponding to the 200/238 bp genotype of microsatellite loci GC101 was the highest,reaching 1.74,which can be used for preliminary marker-assisted selection.

关 键 词：绵羊 FECB 微卫星 繁殖性状 

分 类 号：S826[农业科学—畜牧学]