安石榴苷促进成骨治疗绝经后骨质疏松  

Punicalagin treats postmenopausal osteoporosis by promoting osteogenesis

作　　者：张树东 黄一琳 姚琦 Zhang Shudong;Huang Yilin;Yao Qi(Second Department of Orthopedics,The First Affiliated Hospital of Henan University,Kaifeng 475000,Henan Province,China;Department of Joint,Beijing Shijitan Hospital,Capital Medical University,Beijing 100038,China)

机构地区：[1]河南大学第一附属医院骨二科,河南省开封市475000 [2]首都医科大学附属北京世纪坛医院关节科,北京市100038

出　　处：《中国组织工程研究》2024年第26期4101-4105,共5页Chinese Journal of Tissue Engineering Research

基　　金：河南省医学教育研究项目(Wjlx2021388),项目负责人:黄一琳。

摘　　要：背景:安石榴苷作用广泛,安全性高,但其对成骨细胞和绝经后骨质疏松症的作用尚不清楚。目的:探讨安石榴苷对成骨细胞和绝经后骨质疏松症的作用。方法:安石榴苷作用于MC3T3-E1细胞,检测其促成骨细胞增殖作用。在成骨诱导液中添加安石榴苷,检测其促成骨分化作用。卵巢切除大鼠术后灌胃安石榴苷,3个月后进行MicroCT扫描和血清1型前胶原氨基末端肽检测,观察治疗效果。结果与结论:①CCK-8检测发现安石榴苷能促进成骨细胞增殖(P<0.05);②qRT-PCR和Western blot检测发现安石榴苷能促进碱性磷酸酶、Runx2 mRNA和蛋白的表达(P<0.05);③Micro CT扫描和血清学检测结果显示,安石榴苷能改善卵巢切除大鼠的骨密度、骨体积分数、骨小梁厚度、骨小梁数目和1型前胶原氨基末端肽水平;④结果表明,安石榴苷能促进成骨细胞增殖和分化,且对绝经后骨质疏松大鼠有治疗作用。BACKGROUND:Punicalagin has a wide range of effects and high safety,but its effect on osteoblasts and postmenopausal osteoporosis is unknown.OBJECTIVE:To investigate the effect of punicalagin on osteoblasts and postmenopausal osteoporosis.METHODS:The effect of punicalagin on the proliferation of MC3T3-E1 cells was detected.Punicalagin was added to the osteogenic induction medium to detect its effect on osteogenic differentiation.Punicalagin was used to treat ovariectomized rats and Micro CT scan and serum procollagen type 1 N-terminal propeptide test were performed after 3 months to detect the therapeutic effect.RESULTS AND CONCLUSION:Cell counting kit-8 assay showed that punicalagin could promote the proliferation of osteoblasts(P<0.05).The results of qRT-PCR and western blot showed that punicalagin could promote the mRNA and protein expressions of alkaline phosphatase and Runx2 in osteoblasts(P<0.05).The results of Micro CT scan and serological test showed that punicalagin could improve bone mineral density,bone volume fraction,trabecular thickness,trabecular number and procollagen type 1 N-terminal propeptide level of ovariectomized rats.To conclude,punicalagin can promote osteoblast proliferation and differentiation,and have therapeutic effects in postmenopausal osteoporosis rats.

关 键 词：安石榴苷 骨质疏松症 卵巢切除 成骨细胞 1型前胶原氨基末端肽 

分 类 号：R453.9[医药卫生—治疗学] R336[医药卫生—临床医学] R681