“筋出槽”大鼠模型骨骼肌形态学和功能的特征性变化  

Characteristic changes in morphology and function of skeletal muscles in a rat model of“tendon off-position”

作　　者：杨宗睿 葛海雅 石金玉 汪正明 王媛媛 李正言[1,2] 杜国庆 詹红生[1,2] Yang Zongrui;Ge Haiya;Shi Jinyu;Wang Zhengming;Wang Yuanyuan;Li Zhengyan;Du Guoqing;Zhan Hongsheng(Shi’s Center of Orthopedics and Traumatology,Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China;Institute of Traumatology&Orthopedics,Shanghai Academy of Traditional Chinese Medicine,Shanghai 201203,China)

机构地区：[1]上海中医药大学附属曙光医院石氏伤科医学中心,上海市201203 [2]上海市中医药研究院骨伤科研究所,上海市201203

出　　处：《中国组织工程研究》2024年第26期4170-4177,共8页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金项目(82074466),项目负责人:詹红生;詹红生全国名老中医药专家传承工作室建设项目(国中医药人教函[2022]75号),项目负责人:詹红生;上海市慢性筋骨病临床医学研究中心(20MC1920600),项目负责人:詹红生;上海市临床重点专科“中医骨伤科”(shslczdzk03901),项目负责人:詹红生;上海高水平地方高校“慢性筋骨病损研究与转化”创新团队(沪教委人[2022]3号),项目负责人:詹红生。

摘　　要：背景:“筋出槽”是被《国际疾病分类第十一次修订本》收录的疾病名称,也是手法、针刺等治疗的临床指征,但其具体的效应机制尚不明确,亟待建立一种可以反映其临床特征和病理特点的动物模型,以便深入研究其临床治疗方法的作用机制。目的:基于骨骼肌等长收缩建立大鼠“筋出槽”疾病动物模型,探究“筋出槽”后大鼠骨骼肌形态学和功能的特征性变化。方法:60只大鼠随机分为对照组、静态负荷组和额外负重组,每组20只。对照组大鼠正常饲养,不做处理;后两组采用作者课题组设计的静态负荷悬挂造模装置固定大鼠,通过施加静态负荷(以大鼠自身体质量作为静态负荷)使大鼠上肢肌肉持续的等长收缩,建立“筋出槽”损伤模型;额外负重组在静态负荷组的基础上于大鼠踝关节处额外施加体质量50%的质量。分别于造模2,4周后取材,测试大鼠四肢抓力、骨骼肌湿质量、血清肌肌酸激酶和乳酸脱氢酶A水平的改变情况,观察大鼠骨骼肌组织形态学和超微结构的变化。结果与结论:①2周时,模型组(静态负荷组和额外负重组)大鼠抓力显著降低,骨骼肌湿质量显著下降,血清肌肌酸激酶和乳酸脱氢酶A水平显著上升,肌纤维形态结构异常,伴有大量胶原纤维沉积,电镜结果显示肌原纤维结构紊乱,Z线扭曲,明暗界限模糊;②4周时,模型组大鼠抓力较2周有所上升,血清肌肌酸激酶和乳酸脱氢酶A水平下降,肌纤维形态和超微结构的改变均有不同程度恢复;③提示基于骨骼肌持续等长收缩建立的大鼠骨骼肌损伤模型,2周时可良好反映“筋出槽”的病理特点,可用于进行针刺、手法等治疗“筋出槽”的效应机制研究。BACKGROUND:“Tendon off-position”is a disease name included in the International Classification of Diseases 11th Revision,and also a clinical indication of manipulation,acupuncture and other treatments.However,its specific mechanism is still unclear.It is urgent to establish an animal model that can reflect the clinical and pathological characteristics of“tendon off-position,”so as to further study the mechanism of effective clinical treatments.OBJECTIVE:To establish an animal model of“tendon off-position”in rats based on isometric contraction of skeletal muscles,and to explore the changes of skeletal muscle function and morphological phenotype after“tendon off-position.”METHODS:Sixty rats were randomly divided into control group,static-loading group and extra loading group,with twenty rats in each group.Rats in the control group were kept normally without treatment.In the latter two groups,the rats were fixed by the self-made static-loading modeling device and a static-loading(the body mass of each rats was applied as the static-loading)was applied to cause sustained isometric contraction of the upper limb muscles.Then,animal models of“tendon off-position”were successfully established.In the extra loading group,50%of the body mass was added to the ankle joint after modeling.The skeletal muscle samples were harvested at 2 and 4 weeks after modeling.The changes of limb grip strength,wet mass of skeletal muscle,and serum levels of creatine kinase-muscle and lactate dehydrogenase A were measured,and the changes of skeletal muscle histomorphology and ultrastructure were observed.RESULTS AND CONCLUSION:At 2 weeks after modeling,the rats in the static-loading group and extra loading group showed significantly decreased grip strength and wet muscle mass,significantly increased serum levels of creatine kinase-muscle and lactate dehydrogenase A,and abnormal muscle fiber morphology and structure accompanied by a large number of deposited collagen fibers.Electron microscopy results showed that the structu

关 键 词：“筋出槽” 中医证候 骨骼肌 等长收缩 肌酸激酶 乳酸脱氢酶 动物模型 

分 类 号：R459.9[医药卫生—治疗学] R318[医药卫生—临床医学] R274