髓内固定构建大鼠股骨骨不连模型  

Establishment of a rat femoral nonunion model by intramedullary fixation

作　　者：吕利军 彭伟 李闯兵 叶铄 高秋明 Lyu Lijun;Peng Wei;Li Chuangbing;Ye Shuo;Gao Qiuming(The First Clinical Medical School of Gansu University of Chinese Medicine,Lanzhou 730000,Gansu Province,China;Orthopedic Center of the 940^(th)Hospital of China People’s Liberation Army Joint Logistics Support Force,Lanzhou 730050,Gansu Province,China;Department of Rehabilitation,Gansu Armed Police Corps Hospital,Lanzhou 730000,Gansu Province,China)

机构地区：[1]甘肃中医药大学第一临床医学院,甘肃省兰州市730000 [2]中国人民解放军联勤保障部队第九四〇医院骨科中心,甘肃省兰州市730050 [3]武警甘肃总队医院康复科,甘肃省兰州市730000

出　　处：《中国组织工程研究》2024年第26期4189-4193,共5页Chinese Journal of Tissue Engineering Research

基　　金：甘肃省自然科学基金资助项目(21JR11RA001),项目负责人:高秋明;甘肃省自然科学基金资助项目(22JR5RA009),项目负责人:叶铄。

摘　　要：背景:建立客观标准的骨不连动物模型是骨不连的实验研究及探索骨不连治疗的必备条件。目的:为骨不连的实验研究建立一种客观的动物模型。方法:选取SPF级雄性Wistar大鼠,采用大鼠股骨中段截除5mm、进行大段骨膜剥离及骨髓去除的方法制备大鼠股骨骨不连模型,使用1.2mm克氏针进行固定。于术后1,4,8周通过大体标本观察、X射线片检查、组织病理学观察骨不连形成情况。结果与结论:造模后8周大体标本及X射线拍片、组织病理学检查显示大鼠骨缺损区无骨痂形成,断端纤维组织填充,骨性骨痂稀少,甚至不可见。结果说明,股骨中段骨质截除、大段骨膜剥离及骨髓去除法能够成功制备大鼠骨不连动物模型,该方法简单、可靠、有效。BACKGROUND:Establishing an objective and standard animal model of bone nonunion is essential for experimental studies and treatment of nonunion.OBJECTIVE:To establish an objective animal model for experimental studies of nonunion.METHODS:Specific pathogen-free male Wistar rats were selected and prepared by cutting off a 5 mm bone defect in the middle femur,peeling off a large periosteum and removing bone marrow.Animal models were fixed with a 1.2 mm Kirschner wire.At 1,4 and 8 weeks,bone nonunion was observed by gross specimen observation,X-ray examination and histopathological examination.RESULTS AND CONCLUSION:The gross specimen,X-ray film and histopathological examination showed that there was no callus formation in the bone defect area,the broken end was filled with fiber tissue,and the bone callus was rare or even invisible.To conclude,the rat model of nonunion can be successfully established by osteotomy of the middle femur,large periosteum peeling and bone marrow removal.This modeling method is simple,reliable and effective.

关 键 词：骨不连 动物模型 股骨 放射学 病理 

分 类 号：R496[医药卫生—康复医学] R318[医药卫生—临床医学] R683