Using phylogenomics to untangle the taxonomic incongruence of yellow-flowered Camellia species(Theaceae)in China  

作　　者：Su-Juan Wei Yong-Qing Liufu He-Wen Zheng Hai-Ling Chen Yan-Chi Lai Yan Liu Quan-Qing Ye Shao-Qing Tang 

机构地区：[1]Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection,Ministry of Education,Guangxi Normal University,Guilin 541004,Guangxi,China [2]State Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Resources,School of Life Sciences,Sun Yat-Sen University,Guangzhou 510275,China [3]Guangxi Museum of Natural History,Nanning 530012,China [4]Guangxi Institute of Botany,Guangxi Zhuang Autonomous Region and Chinese Academy of Sciences,Guilin 541006,Guangxi,China

出　　处：《Journal of Systematics and Evolution》2023年第5期748-763,共16页植物分类学报（英文版）

基　　金：funded by the National Natural Science Foundation of China(grant number 31760088);the Biodiversity Survey,Observation and Assessment Program of Ministry of Ecology and Environment of China(grant number 83728);Key Laboratory of Ecology of Rare Endangered Species and Environmental Protection(Guangxi Normal University);Ministry of Education,China(grant number ERESEP2021Ko4).

摘　　要：All yellow Camellia plants in China are threatened,but their classification is contentious.Here,we performed a phylogenetic reconstruction based on nuclear double-digest restriction site-associated DNA sequencing(ddRAD),transcriptomes,nuclear ribosomal internal transcribed spacer(nrITS),and the small single-copy region of the chloroplast genome,in combination with morphological evidence to help resolve taxonomic ambiguity of those rare and threatened species.Conflicting relationships were derived from nuclear and chloroplast sequences.The strong hybridization/introgression signal detected suggests reticulate evolution mainly caused this discordance pattern.The nuclear-ddRAD and RNA-seq phylogenies fit better to the yellow camellias'morphology,providing a clear resolution for inferring their relationships in China.Based on present phylogenetic analyses and morphological characters,we propose these taxonomic suggestions:(i)Camellia petelotii and Camellia nitidissima are distinct species;(ii)Camellia ptilosperma,Camellia longruiensis,Camellia longgangensis,and C.longgangensis var.grandis are conspecific with Camellia flavida;(iii)Camellia multipetala and C.longgangensis var.patens are synonyms of Camellia quinqueloculosa,and C.quinqueloculosa should be recognized as an independent species;(iv)Camellia wumingensis should be formally recognized as an independent species;(v)Camellia longzhouensis is a synonym of Camellia chrysanthoides;(vi)Camellia xiashiensis and Camellia parvipetala should be treated as synonyms of Camellia micrantha;(vii)Camellia achrysantha is a good species;(viii)Camellia tunghinensis is an independent species;(ix)species status of Camellia huana is well-supported;and(x)Camellia pingguoensis var.terminals should be correctly identified to species level as Camellia terminalis.These results provide the basis for the recognition 20 yellow Camellia species in China.

关 键 词：chloroplast DNA sequence ddRAD hybridization NRITS taxonomy transcriptome yellow camellias 

分 类 号：Q94[生物学—植物学]