鹅星状病毒感染抗体依赖性增强作用  

Study on antibody-dependent enhancement during goose astrovirus infection

作　　者：赵光伟 邵骜骏[1] 曹瑞兵 曹蓝云[2] 冯昊南 曹礼静 谭昌藩 杨晓伟 魏思远 张天奇[1] 李军 崔晓焓 张立武 ZHAO Guangwei;SHAO Aojun;CAO Ruibing;CAO Lanyun;FENG Haonan;CAO Lijing;TAN Changfan;YANG Xiaowei;WEI Siyuan;ZHANG Tianqi;LI Jun;CUI Xiaohan;ZHANG Liwu(Chongqing Sanjiezhongxin Bio-engineering Co.,Ltd.,Chongqing 402460,China;College of Veterinary Medicine,Southwest University,Chongqing 402460,China;College of Veterinary Medicine,Nanjing Agricultural University,Nanjing 210095,China;Chongqing Rongchang Vocational Education Center,Chongqing 402460,China;Chongqing Wushan Vocational Education Center,Chongqing 404700,China;Chongqing Hesheng Bio-tech Co.,Ltd.,Chongqing 402460,China)

机构地区：[1]重庆三杰众鑫生物工程有限公司,重庆402460 [2]西南大学动物医学学院,重庆402460 [3]南京农业大学动物医学学院,江苏南京210095 [4]重庆市荣昌区职业教育中心,重庆402460 [5]重庆市巫山县职业教育中心,重庆404700 [6]重庆合盛生物技术有限公司,重庆402460

出　　处：《中国兽医学报》2023年第6期1181-1186,共6页Chinese Journal of Veterinary Science

基　　金：重庆市技术创新与应用示范专项重点示范资助项目(Cstc2018jszx-cyzdX0043)。

摘　　要：为了探究鹅星状病毒感染是否存在抗体依赖性增强作用,用中和效价为1∶94、1∶141和1∶282共3种不同抗体滴度(低、中、高效价)的鹅星状病毒卵黄抗体分别进行雏鹅保护试验和鹅胚中和试验,同时设空白对照和攻毒对照。结果显示,雏鹅保护试验中抗体效价1∶94组鹅均发病,病死率90%(9/10),1∶141效价组鹅的发病率为30%(3/10),病死率20%(2/10),1∶282效价组鹅无发病及死亡;攻毒对照组鹅100%发病,病死率80%(8/10);1∶94抗体组动物发病时间和死亡时间均早于攻毒对照组;荧光定量PCR检测结果显示1∶94和1∶141抗体效价组Ct值均小于攻毒对照组。鹅胚中和试验结果显示,3个抗体处理组鹅胚病死率分别为100%,20%和0,攻毒和阴性对照组分别为100%和0,1∶94效价组鹅胚死亡时间显著早于攻毒对照组;荧光定量检测结果显示1∶94效价组平均Ct值显著小于1∶141效价组和攻毒对照组。结果说明低中和效价的鹅星状病毒卵黄抗体能增强鹅星状病毒在雏鹅和鹅胚体内的复制能力,发病率和病死率增加,证实鹅星状病毒感染存在抗体依赖性增强作用,其结果可为临床鹅星状病毒病的防控提供理论依据。In order to explore the existence of antibody-dependent enhancement effect(ADE)during goose astrovirus infection,goose protection test and goose embryo neutralization test were carried out with goose astrovirus yolk antibodies with neutralization titers of 1:94,1:141 and 1:282(low,medium and high titers),respectively.Meanwhile,blank control and challenge control groups were set.The results of gosling protection test showed that the geese in the antibody titer 1:94 group were all sick,and the mortality rate was 90%(9/10),while the geese in the antibody titer 1:141 group had a incidence rate of 30%(3/10)and the mortality rate was 20%(2/10).However,the geese in the antibody titer 1:282 group had no morbidity and mortality.The geese in the challenge control group were 100%sick and 80%dead(8/10).From the dead time of the animals in these groups,the 1:94 antibody group were 3 d earlier than that of the challenge control group.Results of the fluorescence quantitative PCR test showed that the titers of the 1:94 and 1:141 antibody groups were less than that of the control group,indicating that their viral load was higher.The results of goose embryo neutralization test showed that the death rates of goose embryos in the three groups of antibody treatment groups were 100%,20%and O,respectively.While in the challenge and negative control groups were 10o%and O,respectively.The death time of duck embryos in the 1:94 titer group was significantly earlier than that in the challenge control group.The average C,values of fluorescence quantitative PCR detection of samples in the five groups were 16.803,31.609,0.000,0.000 and 25.375,respectively.It could be found that the 1:94 titer group was significantly lower than that in the 1:141 titer group and the challenge control group.Combined with the results of the two experiments,it was showed that the egg yolk antibody of goose astrovirus with low neutralization titer(1:94)could enhance the replication of goose astrovirus in goslings and goose embryos,and increase the incidence rate an

关 键 词：鹅星状病毒 雏鹅试验 鹅胚中和试验 抗体依赖性增强作用 

分 类 号：S852.4[农业科学—基础兽医学] S852.65[农业科学—兽医学]