褪黑素介导的经皮耳迷走神经电刺激降糖效应机制  

Mechanism of melatonin-mediated antihyperglycemic effect of transcutaneous auricular vagus nerve stimulation

作　　者：张煜郑姮 辛陈 张紫璇 张凯琪 李亮[1] 荣培晶[1] 李少源[1] ZHANG Yu-zheng-heng;XIN Chen;ZHANG Zi-xuan;ZHANG Kai-qi;LI Liang;RONG Pei-jing;LI Shao-yuan(Institute of Acupuncture and Moxibustion,China Academy of Chinese Medical Sciences,Beijing 100700,China;Wangjing Hospital,China Academy of Chinese Medical Sciences,Beijing 100102)

机构地区：[1]中国中医科学院针灸研究所,北京100700 [2]中国中医科学院望京医院,北京100102

出　　处：《针刺研究》2023年第8期812-817,共6页Acupuncture Research

基　　金：国家自然科学基金青年基金项目(No.82004181);中国科协青年人才托举工程(No.2021-2023ZGZJXH-QNRC002);中国中医科学院基本科研业务费优秀青年科技人才培养专项(No.ZZ15-YQ-048)。

摘　　要：目的:观察经皮耳迷走神经电刺激(taVNS)对Zucker糖尿病肥胖(ZDF)大鼠血浆褪黑素(MLT)含量及肝脏、骨骼肌、胰腺胰岛素受体表达的影响,探讨taVNS的降糖机制。方法:30只ZDF雄性大鼠随机分为模型组、经皮耳迷走神经电刺激组(taVNS组)、假taVNS组,每组10只,另以10只同种系雄性Zucker瘦型大鼠作为空白对照组。ZDF大鼠采用高脂饲料喂养诱导2型糖尿病大鼠模型。taVNS组使用HANS-100A型电针仪刺激大鼠双侧耳甲腔,假taVNS组刺激部位与taVNS组相同,但不通电。以上干预均每日1次,30 min/次,连续6周。每周测量1次各组大鼠空腹血糖(FBG);ELISA法检测大鼠血浆MLT含量;Western blot法检测大鼠肝脏、骨骼肌、胰腺组织中胰岛素受体表达水平。结果:与空白对照组比较,模型组大鼠FBG升高(P<0.01),血浆MLT含量降低(P<0.01),胰腺组织中胰岛素受体表达水平降低(P<0.01)。与模型组比较,taVNS组大鼠FBG降低(P<0.05,P<0.01),血浆MLT含量升高(P<0.01),肝脏、骨骼肌、胰腺组织中胰岛素受体表达水平均升高(P<0.05,P<0.01,P<0.001)。与taVNS组比较,假taVNS组大鼠FBG升高(P<0.05,P<0.01),血浆MLT含量降低(P<0.01),骨骼肌和胰腺组织中胰岛素受体表达水平均降低(P<0.01,P<0.001)。结论:taVNS可能是通过调节MLT含量,改善胰岛素抵抗,最终实现其降糖效应。Objective To observe the effects of transcutaneous auricular vagus nerve stimulation(taVNS) on plasma melatonin(MLT) content and insulin receptor expression in the liver,the skeletal muscles,and the pancreas of Zucker diabetic fatty(ZDF) rats,so as to explore the hypoglycemic mechanism of taVNS.Methods Thirty male ZDF rats were randomly divided into model group,taVNS group and sham-taVNS group,with 10 rats in each group;besides,10 male Zucker lean rats of the same strain were collected for the blank control group.ZDF rats were fed with high-fat diet to induce type 2 diabetes mellitus(T2DM) rat model.In the taVNS group,HANS-100A electroacupuncture instrument was used to stimulate the cavum conchae of both sides.The stimulation sites of rats in the sham-taVNS were the same as the taVNS group,but without electricity delivered.The above interventions were performed 30 min each time,once daily,lasting for 6 weeks.Fasting blood glucose(FBG) was measured weekly in each group,the plasma metatonin(MLT) content was detected by ELISA,and the insulin receptor expression level in the liver,the skeletal muscle and the pancreas was determined by Western blot.Results Compared with the blank control group,the level of FBG of rats were increased(P<0.01),the plasma MLT content was decreased(P<0.01) and the insulin receptor expression level in the pancreatic tissue was decreased(P<0.01) in the model group.In the taVNS gruop,FBG was decreased(P<0.05,P<0.01),the plasma MLT content was increased(P<0.01),and the insulin receptor expression level in the liver,the skeletal muscle and the pancreas was increased(P<0.05,P<0.01,P<0.001) when compared with the model group.Compared with the taVNS group,FBG was increased(P<0.05,P<0.01),the plasma MLT content was decreased(P<0.01),and the expression level of insulin receptors in the skeletal muscle and the pancreas was decreased(P<0.01,P<0.001) in the sham-taVNS group.Conclusion The taVNS can improve the insulin resistance and ultimately obtain the antihyperglycemic effect through regulating MLT

关 键 词：经皮耳迷走神经电刺激 2型糖尿病 褪黑素 迷走神经 

分 类 号：R246.1[医药卫生—针灸推拿学]