microRNA-27b、microRNA-221、microRNA-222和血管内皮生长因子在瘢痕疙瘩中的表达及相关性研究  

Expression and correlation of microRNA-27b, microRNA-221, microRNA-222 and vascular endothelial growth factor in keloids

作　　者：朱学娥 向芳[1] 罗东[1] 康晓静[1] 丁媛[1] Zhu Xue’e;Xiang Fang;Luo Dong;Kang Xiaojing;Ding Yuan(Department of Dermatology and Venereology,People’s Hospital of Xinjiang Uygur Autonomous Region,Xinjiang Clinical Research Center for Dermatologic Diseases,Xinjiang Key Laboratory of Dermatology Research(XJYS1707),Urumqi 830001,China)

机构地区：[1]新疆维吾尔自治区人民医院皮肤性病科、新疆皮肤病临床医学研究中心、新疆皮肤病研究重点实验室(XJYS1707),乌鲁木齐830001

出　　处：《中华整形外科杂志》2023年第8期844-850,共7页Chinese Journal of Plastic Surgery

基　　金：新疆维吾尔自治区自然科学基金资助项目(2019D01C134,2021D01C147)。

摘　　要：目的探讨瘢痕疙瘩中microRNA-27b(miR-27b)、miR-221和miR-222的表达及与血管内皮生长因子(VEGF)的关系。方法选取2021年1月至2022年1月新疆维吾尔自治区人民医院皮肤性病科手术切除的瘢痕疙瘩组织(瘢痕疙瘩组)及对应瘢痕旁正常组织(正常对照组)各36例,另选择同期符合诊断标准的扁平瘢痕(扁平瘢痕组)12例。采用免疫组织化学法测定3组中VEGF蛋白表达情况和微血管密度,通过实时荧光定量PCR检测miR-27b、miR-221和miR-222表达水平。采用SPSS 27.0统计软件进行分析,正态分布的计量资料以x±s表示,多组间比较采用单因素方差分析,两两多重比较采用LSD法;非正态分布的计量资料以M(Q_(1),Q_(3))表示,组间比较采用Kruskal-WallisH检验;数据间的相关性分析采用Spearman相关分析法,所有检验均为双侧检验,P<0.05为差异有统计学意义。结果瘢痕疙瘩组VEGF蛋白表达水平和微血管计数[0.28±0.08、(47.78±14.06)条/400倍视野]明显高于正常对照组[0.09±0.05、(10.25±5.08)条/400倍视野]和扁平瘢痕组[0.19±0.06、(23.75±7.94)条/400倍视野],差异有统计学意义(P<0.01)。瘢痕疙瘩组miR-27b表达水平[0.50(0.32,0.64)]显著低于正常对照组[0.69(0.37,1.69)]和扁平瘢痕组[1.35(1.25,1.74)],差异有统计学意义(P<0.01);而3组miR-221及miR-222的表达水平差异无统计学意义(P>0.05)。相关性分析显示,瘢痕疙瘩组中miR-27b、miR-221、miR-222与VEGF蛋白表达均呈正相关关系(r=0.36、0.41、0.37,P均<0.05)。结论miR-27b在瘢痕疙瘩、扁平瘢痕及正常组织中表达具有差异性,瘢痕疙瘩中miR-27b、miR-221和miR-222与VEGF表达呈正相关,提示miRNA/VEGF轴可能在瘢痕疙瘩发生和进展中起到关键性作用。Objective To investigate the expression of microRNA-27b(miR-27b),miR-221 and miR-222 in keloids and their relationships with vascular endothelial growth factor(VEGF).Methods From January 2021 to January 2022,36 cases of keloid tissue(keloid group),36 cases of adjacent normal tissue(normal control group)and 12 cases of flat scars(flat scar group)were collected from Dermatology and Venereology Department of Xinjiang Uygur Autonomous Region People's Hospital.VEGF protein expression and microvessel density in the three groups were determined by immunohistochemistry,and the expression levels of miR-27b,miR-221 and miR-222 were detected by real-time quantitative PCR.SPSS 27.0 statistical software was used for statistical analysis.The normally distributed measurement data were expressed as Mean±SD.ANOVA was used for multi-group comparison,and the LSD method was used for pound-for-pair multiple comparisons.The measurement data of non-normal distribution were expressed as M(Q_(1),Q_(3)),and Kruskal-Wallis H test was used for the comparison between groups.Spearman correlation analysis was used for the correlation analysis among data.All tests were bilateral tests,and P<0.05 was considered statistically significant.Results The levels of VEGF protein and microvessel count in the keloid group[0.28±0.08,(47.78±14.06)bar/×400 view]were significantly higher than those in normal control group[0.09±0.05,(10.25±5.08)bar/×400 view]and flat scar group[0.19±0.06,(23.75±7.94)bar/×400 view],the difference was statistically significant(P<0.01).The expression level of miR-27b in keloid group[0.50(0.32,0.64)]was significantly lower than that in normal control group[0.69(0.37,1.69)]and flat scar group[1.35(1.25,1.74)],the difference was statistically significant(P<0.01).There were no significant differences in the expression levels of miR-221 and miR-222 among the three groups(P>0.05).Correlation analysis showed that miR-27b,miR-221,miR-222 were positively correlated with VEGF protein expression in keloid group(r=0.36,0.41,0.37,P<

关 键 词：瘢痕疙瘩 微RNAS 血管内皮生长因子 相关性分析 

分 类 号：R622[医药卫生—整形外科]